miRNA: 3'- caAGGGCaACaCGGCGAAG-------GCUUGGc -5'
miRNA: 3'- caaGGGCAACa-----CGgCGaAGGCUUGGC- -5'
miRNA: 3'- caaGGGCAACaCGGCGaaGG----------CUUGGC- -5'
miRNA: 3'- -CAAGGG-CAACACGgCGaa--GGCUUGGC- -5'
miRNA: 3'- -CAAGGGCAACACGGCGAAGGCUUGGC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1451 | 3' | -54.7 | NC_001335.1 | + | 25181 | 0.66 | 0.753128 |
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Target: 5'- -aUCUCGaUGaGCCGCUUCucgcccgCGAGCCa -3' miRNA: 3'- caAGGGCaACaCGGCGAAG-------GCUUGGc -5' |
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| 1451 | 3' | -54.7 | NC_001335.1 | + | 18063 | 0.67 | 0.690114 |
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Target: 5'- --aCCCGUUGgugaagGCaGCcUCCGGGCUGa -3' miRNA: 3'- caaGGGCAACa-----CGgCGaAGGCUUGGC- -5' |
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| 1451 | 3' | -54.7 | NC_001335.1 | + | 25926 | 0.68 | 0.612969 |
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Target: 5'- --cCCCGUUG-GCCGCagCCagcucgcugaGAGCCGu -3' miRNA: 3'- caaGGGCAACaCGGCGaaGG----------CUUGGC- -5' |
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| 1451 | 3' | -54.7 | NC_001335.1 | + | 50109 | 0.73 | 0.360926 |
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Target: 5'- aGUUCCCaGUUGUGCaGCaggaCCGAGCUGa -3' miRNA: 3'- -CAAGGG-CAACACGgCGaa--GGCUUGGC- -5' |
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| 1451 | 3' | -54.7 | NC_001335.1 | + | 9021 | 1.1 | 0.000972 |
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Target: 5'- cGUUCCCGUUGUGCCGCUUCCGAACCGg -3' miRNA: 3'- -CAAGGGCAACACGGCGAAGGCUUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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