miRNA: 3'- gUUCUCGGa---CGugCACaCGUGG-UGau -5'
miRNA: 3'- -GUUCUCGG--------ACGUGCACAcGUGGUGau -5'
miRNA: 3'- gUUCUCgGACGuGCACACG-UGGUGau -5'
miRNA: 3'- -GUUCUCGGACGUGcAC--ACGUGGUGau -5'
miRNA: 3'- -GUUCUCGGACGUGcACaCGUGGuGAU- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14528 | 3' | -55 | NC_003521.1 | + | 20639 | 0.7 | 0.820312 |
|
Target: 5'- gGAGAGCCgcgcGCugGUGgGCGCCuACg- -3' miRNA: 3'- gUUCUCGGa---CGugCACaCGUGG-UGau -5' |
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| 14528 | 3' | -55 | NC_003521.1 | + | 118943 | 0.7 | 0.811668 |
|
Target: 5'- cCAGGAGCCcaugucgcUGUACGUGUaCGCCAUg- -3' miRNA: 3'- -GUUCUCGG--------ACGUGCACAcGUGGUGau -5' |
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| 14528 | 3' | -55 | NC_003521.1 | + | 18495 | 0.71 | 0.756691 |
|
Target: 5'- gAGGAG-CUGCcCGUGUGCcACCACa- -3' miRNA: 3'- gUUCUCgGACGuGCACACG-UGGUGau -5' |
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| 14528 | 3' | -55 | NC_003521.1 | + | 213738 | 0.75 | 0.575828 |
|
Target: 5'- uCGAGAGCCUGCAC-UGccUGCGCUACc- -3' miRNA: 3'- -GUUCUCGGACGUGcAC--ACGUGGUGau -5' |
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| 14528 | 3' | -55 | NC_003521.1 | + | 140644 | 0.76 | 0.477826 |
|
Target: 5'- cCGAGAGCCUGCGCcUG-GCGCC-CUAc -3' miRNA: 3'- -GUUCUCGGACGUGcACaCGUGGuGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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