miRNA: 3'- -CCGCACCACuu------------GCCauuUACCGGGUG- -5'
miRNA: 3'- cCG-CACCACU-------------UGCCauuuaCCGGGUG- -5'
miRNA: 3'- -CCGCACCACUUGC-CAuuua-------CCGGGug -5'
miRNA: 3'- -CCGCAC--CACUUg----CCAUUU-------ACCGgGUG- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14547 | 5' | -53 | NC_003521.1 | + | 18329 | 0.67 | 0.980337 |
|
Target: 5'- cGGCGUGGUGcuguuggaccaguuCGGc--GUGGUCUACu -3' miRNA: 3'- -CCGCACCACuu------------GCCauuUACCGGGUG- -5' |
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| 14547 | 5' | -53 | NC_003521.1 | + | 18260 | 0.7 | 0.934892 |
|
Target: 5'- aGCgGUGGUGGggaccugcccgcgACGGgag--GGCCCGCa -3' miRNA: 3'- cCG-CACCACU-------------UGCCauuuaCCGGGUG- -5' |
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| 14547 | 5' | -53 | NC_003521.1 | + | 12357 | 0.68 | 0.973944 |
|
Target: 5'- gGGCGUGGUGGGCGaGUcgcuacccuccGGCCUc- -3' miRNA: 3'- -CCGCACCACUUGC-CAuuua-------CCGGGug -5' |
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| 14547 | 5' | -53 | NC_003521.1 | + | 368 | 0.67 | 0.981985 |
|
Target: 5'- cGGCGUGcuGUGGAacgaaGGUGAGugugugcUGGCgCGCg -3' miRNA: 3'- -CCGCAC--CACUUg----CCAUUU-------ACCGgGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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