miRNA: 3'- -CCGCACCACUUGC-CAuuua-------CCGGGug -5'
miRNA: 3'- -CCGCACCaCU-UGCCaUUUa----CCG--GGUG- -5'
miRNA: 3'- cCGCaCCaCUUGCCa---UUuaCCGGGUG- -5'
miRNA: 3'- -CCGCACC------ACUu--GCCAUuuaCCGGGUG- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14547 | 5' | -53 | NC_003521.1 | + | 12357 | 0.68 | 0.973944 |
|
Target: 5'- gGGCGUGGUGGGCGaGUcgcuacccuccGGCCUc- -3' miRNA: 3'- -CCGCACCACUUGC-CAuuua-------CCGGGug -5' |
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| 14547 | 5' | -53 | NC_003521.1 | + | 98227 | 0.68 | 0.97548 |
|
Target: 5'- cGGCGUGG-GAgGCGGgGAGgcgggGGCggCCACg -3' miRNA: 3'- -CCGCACCaCU-UGCCaUUUa----CCG--GGUG- -5' |
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| 14547 | 5' | -53 | NC_003521.1 | + | 103473 | 0.68 | 0.97548 |
|
Target: 5'- uGCG-GGaGGGCGGcuccAGcgGGCCCGCg -3' miRNA: 3'- cCGCaCCaCUUGCCa---UUuaCCGGGUG- -5' |
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| 14547 | 5' | -53 | NC_003521.1 | + | 167052 | 0.74 | 0.775675 |
|
Target: 5'- cGGCGUGGcgcucaUGAcgcCGGUGcc-GGCCCACa -3' miRNA: 3'- -CCGCACC------ACUu--GCCAUuuaCCGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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