Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14571 | 3' | -51.9 | NC_003521.1 | + | 113347 | 0.66 | 0.997093 |
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Target: 5'- uGGUGAcgcgcAUCUCGgcGCUGG-CGGGc- -3' miRNA: 3'- -CCACUc----UAGAGCaaCGAUCaGCCCuu -5' |
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| 14571 | 3' | -51.9 | NC_003521.1 | + | 83990 | 0.66 | 0.996009 |
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Target: 5'- uGGUGuacAUCUCGUUGCUu-UCGuGGAGc -3' miRNA: 3'- -CCACuc-UAGAGCAACGAucAGC-CCUU- -5' |
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| 14571 | 3' | -51.9 | NC_003521.1 | + | 99359 | 0.66 | 0.995355 |
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Target: 5'- cGGUGAGGcugcUgUUGUUGCUGguggucGUCGGcGAAg -3' miRNA: 3'- -CCACUCU----AgAGCAACGAU------CAGCC-CUU- -5' |
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| 14571 | 3' | -51.9 | NC_003521.1 | + | 195842 | 0.66 | 0.994615 |
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Target: 5'- --cGAGAUCUCGcgcgGCagGGuUCGGGGAu -3' miRNA: 3'- ccaCUCUAGAGCaa--CGa-UC-AGCCCUU- -5' |
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| 14571 | 3' | -51.9 | NC_003521.1 | + | 172549 | 0.67 | 0.992753 |
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Target: 5'- cGG-GGGAUCUCGUgccGCUgucccggGGUCGGcGGu -3' miRNA: 3'- -CCaCUCUAGAGCAa--CGA-------UCAGCC-CUu -5' |
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| 14571 | 3' | -51.9 | NC_003521.1 | + | 112078 | 0.67 | 0.989374 |
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Target: 5'- cGGUGgGGAUCagGggcgGCUGG-CGGGAAg -3' miRNA: 3'- -CCAC-UCUAGagCaa--CGAUCaGCCCUU- -5' |
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| 14571 | 3' | -51.9 | NC_003521.1 | + | 211221 | 0.68 | 0.984699 |
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Target: 5'- uGGUGaAGGUCUUGgucagcagGCU-GUUGGGAAu -3' miRNA: 3'- -CCAC-UCUAGAGCaa------CGAuCAGCCCUU- -5' |
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| 14571 | 3' | -51.9 | NC_003521.1 | + | 167386 | 0.68 | 0.978603 |
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Target: 5'- uGGUGccgaaAGAgCUCGUcggcgUGCaGGUCGGGGAa -3' miRNA: 3'- -CCAC-----UCUaGAGCA-----ACGaUCAGCCCUU- -5' |
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| 14571 | 3' | -51.9 | NC_003521.1 | + | 197680 | 1.09 | 0.010371 |
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Target: 5'- cGGUGAGAUCUCGUUGCUAGUCGGGAAg -3' miRNA: 3'- -CCACUCUAGAGCAACGAUCAGCCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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