Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14583 | 3' | -52.6 | NC_003521.1 | + | 119484 | 0.66 | 0.99214 |
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Target: 5'- --gGGGCGUGCAGGGuGAaGccGUUGUc -3' miRNA: 3'- guaCCUGCACGUCCCcCUaCa-UAACA- -5' |
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| 14583 | 3' | -52.6 | NC_003521.1 | + | 31035 | 0.66 | 0.989782 |
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Target: 5'- ---cGugGUGguGGGGGGUGggaggaccacgcUGUUGUu -3' miRNA: 3'- guacCugCACguCCCCCUAC------------AUAACA- -5' |
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| 14583 | 3' | -52.6 | NC_003521.1 | + | 122697 | 0.67 | 0.985247 |
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Target: 5'- uCAUGGGCGUcucgGCGGGGcuGGAggcgGUcgUGg -3' miRNA: 3'- -GUACCUGCA----CGUCCC--CCUa---CAuaACa -5' |
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| 14583 | 3' | -52.6 | NC_003521.1 | + | 103540 | 0.68 | 0.976995 |
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Target: 5'- uCAUGGagGCGUGCGuGGGGAUGa----- -3' miRNA: 3'- -GUACC--UGCACGUcCCCCUACauaaca -5' |
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| 14583 | 3' | -52.6 | NC_003521.1 | + | 94611 | 0.68 | 0.976995 |
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Target: 5'- ---uGAUGaGCAGGGGGAUGUGg--- -3' miRNA: 3'- guacCUGCaCGUCCCCCUACAUaaca -5' |
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| 14583 | 3' | -52.6 | NC_003521.1 | + | 105749 | 0.68 | 0.974485 |
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Target: 5'- uCAUGGugGUGUuGGGGGAg------- -3' miRNA: 3'- -GUACCugCACGuCCCCCUacauaaca -5' |
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| 14583 | 3' | -52.6 | NC_003521.1 | + | 155244 | 0.71 | 0.904623 |
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Target: 5'- cCGUGGugGUGguGGGGGgcGg----- -3' miRNA: 3'- -GUACCugCACguCCCCCuaCauaaca -5' |
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| 14583 | 3' | -52.6 | NC_003521.1 | + | 84850 | 0.73 | 0.847527 |
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Target: 5'- --cGGugGUGUAGGGGGAgacGUAg--- -3' miRNA: 3'- guaCCugCACGUCCCCCUa--CAUaaca -5' |
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| 14583 | 3' | -52.6 | NC_003521.1 | + | 206645 | 0.97 | 0.049179 |
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Target: 5'- aCAUGGACGUGCAGGGGGAUGUuaUGUc -3' miRNA: 3'- -GUACCUGCACGUCCCCCUACAuaACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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