Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14586 | 3' | -49.9 | NC_003521.1 | + | 153515 | 0.66 | 0.998176 |
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Target: 5'- cAGGCgcUCGCUGCgcaCCU-UCUUGAAc -3' miRNA: 3'- -UCCGaaAGCGGUGa--GGAuAGAACUUu -5' |
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| 14586 | 3' | -49.9 | NC_003521.1 | + | 82903 | 0.67 | 0.997404 |
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Target: 5'- uGGCgcgUCGCCACUCUcauccgGUCUgGAc- -3' miRNA: 3'- uCCGaa-AGCGGUGAGGa-----UAGAaCUuu -5' |
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| 14586 | 3' | -49.9 | NC_003521.1 | + | 231816 | 0.67 | 0.996927 |
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Target: 5'- uGGCUUUCuGCCACUgCUGUUUc---- -3' miRNA: 3'- uCCGAAAG-CGGUGAgGAUAGAacuuu -5' |
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| 14586 | 3' | -49.9 | NC_003521.1 | + | 121630 | 0.67 | 0.996379 |
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Target: 5'- uGGCgcagCGCCACgUCCUugGUgUUGAAGa -3' miRNA: 3'- uCCGaaa-GCGGUG-AGGA--UAgAACUUU- -5' |
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| 14586 | 3' | -49.9 | NC_003521.1 | + | 4424 | 0.68 | 0.99504 |
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Target: 5'- cGGCUUUCGCgACUCCcg----GAGAa -3' miRNA: 3'- uCCGAAAGCGgUGAGGauagaaCUUU- -5' |
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| 14586 | 3' | -49.9 | NC_003521.1 | + | 83586 | 0.68 | 0.993326 |
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Target: 5'- uGGUagUCaGCCGCUCCUGggcCUUGAu- -3' miRNA: 3'- uCCGaaAG-CGGUGAGGAUa--GAACUuu -5' |
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| 14586 | 3' | -49.9 | NC_003521.1 | + | 207715 | 1.07 | 0.018645 |
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Target: 5'- aAGGCUUUCGCCACUCCUAUCUUGAAAg -3' miRNA: 3'- -UCCGAAAGCGGUGAGGAUAGAACUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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