miRNA: 3'- gGCCCGCa-CU--UGUCAGGCA-------CGUAGac -5'
miRNA: 3'- gGCCCGcaCUUGUCAGG--CACGUAGAc -5'
miRNA: 3'- -GGC--CCGCA------CUUGUCAGG-CACGUAGAC- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14588 | 5' | -55.1 | NC_003521.1 | + | 218786 | 0.7 | 0.882846 |
|
Target: 5'- aUGGGCGcgGAaaACAGUCCGUcuuaucgGCGUCg- -3' miRNA: 3'- gGCCCGCa-CU--UGUCAGGCA-------CGUAGac -5' |
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| 14588 | 5' | -55.1 | NC_003521.1 | + | 224440 | 0.7 | 0.8549 |
|
Target: 5'- gUGGGC--GAGCGGUCCagGUGCGUCUu -3' miRNA: 3'- gGCCCGcaCUUGUCAGG--CACGUAGAc -5' |
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| 14588 | 5' | -55.1 | NC_003521.1 | + | 228041 | 0.71 | 0.847257 |
|
Target: 5'- gUCGguGGCGUuaagcgGAGCGGUUCgGUGCGUCUGg -3' miRNA: 3'- -GGC--CCGCA------CUUGUCAGG-CACGUAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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