Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 14589 | 3' | -61.8 | NC_003521.1 | + | 136874 | 0.66 | 0.768057 |
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Target: 5'- aCUCGCGGCGGguggcgccggUGUUCgCGGucgacaacGACGUg -3' miRNA: 3'- cGAGCGCCGCC----------ACAGGgGCCu-------CUGCA- -5' |
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| 14589 | 3' | -61.8 | NC_003521.1 | + | 164944 | 0.66 | 0.801992 |
|
Target: 5'- cCUC-CGGCGGcucgcGUCgCCGGAGAgGg -3' miRNA: 3'- cGAGcGCCGCCa----CAGgGGCCUCUgCa -5' |
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| 14589 | 3' | -61.8 | NC_003521.1 | + | 124257 | 0.66 | 0.810165 |
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Target: 5'- cGCcgCGCGGCaugcuuGGUGUCUgCGGGcGCGa -3' miRNA: 3'- -CGa-GCGCCG------CCACAGGgGCCUcUGCa -5' |
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| 14589 | 3' | -61.8 | NC_003521.1 | + | 113574 | 0.66 | 0.768057 |
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Target: 5'- aGgaCGCGGUGGggcugGgCCUCGGGGGCa- -3' miRNA: 3'- -CgaGCGCCGCCa----CaGGGGCCUCUGca -5' |
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| 14589 | 3' | -61.8 | NC_003521.1 | + | 115917 | 0.66 | 0.768057 |
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Target: 5'- aGCUCGCGGUagaGGUGaggCCaguaCGGuugGGGCGUc -3' miRNA: 3'- -CGAGCGCCG---CCACa--GGg---GCC---UCUGCA- -5' |
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| 14589 | 3' | -61.8 | NC_003521.1 | + | 125843 | 0.66 | 0.768057 |
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Target: 5'- gGCUCGCGGUGGUcGUacagcgCCuuGGuGGCc- -3' miRNA: 3'- -CGAGCGCCGCCA-CA------GGggCCuCUGca -5' |
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| 14589 | 3' | -61.8 | NC_003521.1 | + | 167680 | 0.66 | 0.785259 |
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Target: 5'- uGCUCGCGGUugcGGUGacgacgCUCguggaGGAGGCGg -3' miRNA: 3'- -CGAGCGCCG---CCACa-----GGGg----CCUCUGCa -5' |
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| 14589 | 3' | -61.8 | NC_003521.1 | + | 91878 | 0.66 | 0.801992 |
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Target: 5'- gGCg-GCGGCGGUGcgaCCaCGGAcgGACGg -3' miRNA: 3'- -CGagCGCCGCCACag-GG-GCCU--CUGCa -5' |
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| 14589 | 3' | -61.8 | NC_003521.1 | + | 181031 | 0.66 | 0.8182 |
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Target: 5'- cGC-CGUGuCGGUGUCCUugCGGaAGGCGg -3' miRNA: 3'- -CGaGCGCcGCCACAGGG--GCC-UCUGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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