Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 14610 | 3' | -50.1 | NC_003521.1 | + | 35210 | 0.66 | 0.999346 |
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Target: 5'- -aGGGGGCGcGCGGG----UCGGAUCg -3' miRNA: 3'- ugCCUCUGCuUGUCCauguAGUCUAG- -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 67866 | 0.66 | 0.999346 |
|
Target: 5'- aGCGGuugGGGCgGAACGGGaggagACGacUCGGAUCu -3' miRNA: 3'- -UGCC---UCUG-CUUGUCCa----UGU--AGUCUAG- -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 72536 | 0.66 | 0.999346 |
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Target: 5'- uGCGGAGGCGGcgGCGacGGUGuCGUCGucUCg -3' miRNA: 3'- -UGCCUCUGCU--UGU--CCAU-GUAGUcuAG- -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 193274 | 0.66 | 0.999346 |
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Target: 5'- uGCGGAG-CGggUgccGGGacggGCGUCGGAgUCg -3' miRNA: 3'- -UGCCUCuGCuuG---UCCa---UGUAGUCU-AG- -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 24264 | 0.66 | 0.999097 |
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Target: 5'- uCGGcGGCGAccuggaucucccucgGCAGGUcCAUCGGuAUCg -3' miRNA: 3'- uGCCuCUGCU---------------UGUCCAuGUAGUC-UAG- -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 49967 | 0.66 | 0.999573 |
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Target: 5'- -aGGAgGACGAgcgGCGGGUGgGUCgagggagccGGGUCg -3' miRNA: 3'- ugCCU-CUGCU---UGUCCAUgUAG---------UCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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