Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14610 | 3' | -50.1 | NC_003521.1 | + | 72536 | 0.66 | 0.999346 |
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Target: 5'- uGCGGAGGCGGcgGCGacGGUGuCGUCGucUCg -3' miRNA: 3'- -UGCCUCUGCU--UGU--CCAU-GUAGUcuAG- -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 182111 | 0.66 | 0.99947 |
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Target: 5'- cUGGAGACGGugGGcUGCGUCAa--- -3' miRNA: 3'- uGCCUCUGCUugUCcAUGUAGUcuag -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 63168 | 0.66 | 0.99947 |
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Target: 5'- cCGGAGACuGGACGuGUGCAacgCAGAg- -3' miRNA: 3'- uGCCUCUG-CUUGUcCAUGUa--GUCUag -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 139512 | 0.66 | 0.99947 |
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Target: 5'- -aGGAGACGGuGCAGGUGuuCAUCGa--- -3' miRNA: 3'- ugCCUCUGCU-UGUCCAU--GUAGUcuag -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 236363 | 0.66 | 0.999573 |
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Target: 5'- uCGGGGGCc-GCAggcGGUGCcuUCAGAUCg -3' miRNA: 3'- uGCCUCUGcuUGU---CCAUGu-AGUCUAG- -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 49967 | 0.66 | 0.999573 |
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Target: 5'- -aGGAgGACGAgcgGCGGGUGgGUCgagggagccGGGUCg -3' miRNA: 3'- ugCCU-CUGCU---UGUCCAUgUAG---------UCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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