Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14610 | 3' | -50.1 | NC_003521.1 | + | 214045 | 0.68 | 0.996628 |
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Target: 5'- cCGGuGGcCGGACAGGUaacugaACAUgAGGUCc -3' miRNA: 3'- uGCCuCU-GCUUGUCCA------UGUAgUCUAG- -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 218040 | 0.71 | 0.970623 |
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Target: 5'- gGCGGcGGCGuccGCAGGUAgAUgAGGUCc -3' miRNA: 3'- -UGCCuCUGCu--UGUCCAUgUAgUCUAG- -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 220751 | 1.09 | 0.016647 |
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Target: 5'- cACGGAGACGAACAGGUACAUCAGAUCc -3' miRNA: 3'- -UGCCUCUGCUUGUCCAUGUAGUCUAG- -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 234837 | 0.71 | 0.973437 |
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Target: 5'- gACGGGGACGGcgAUAGcGUggGCAUCGGcGUCg -3' miRNA: 3'- -UGCCUCUGCU--UGUC-CA--UGUAGUC-UAG- -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 236363 | 0.66 | 0.999573 |
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Target: 5'- uCGGGGGCc-GCAggcGGUGCcuUCAGAUCg -3' miRNA: 3'- uGCCUCUGcuUGU---CCAUGu-AGUCUAG- -5' |
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| 14610 | 3' | -50.1 | NC_003521.1 | + | 238509 | 0.7 | 0.987946 |
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Target: 5'- -aGGAGgccggcGCGAGCAGGUGCGagAGcUCg -3' miRNA: 3'- ugCCUC------UGCUUGUCCAUGUagUCuAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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