Results 61 - 66 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14610 | 3' | -50.1 | NC_003521.1 | + | 32225 | 0.67 | 0.997958 |
Target: 5'- cGCGGcccAGACGGcCAGGUcccacucgaGCGUCAGGc- -3' miRNA: 3'- -UGCC---UCUGCUuGUCCA---------UGUAGUCUag -5' |
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14610 | 3' | -50.1 | NC_003521.1 | + | 28091 | 0.7 | 0.984648 |
Target: 5'- gGCGGGGAagcCGAGCAGGcGCucgcccaAGAUCg -3' miRNA: 3'- -UGCCUCU---GCUUGUCCaUGuag----UCUAG- -5' |
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14610 | 3' | -50.1 | NC_003521.1 | + | 24264 | 0.66 | 0.999097 |
Target: 5'- uCGGcGGCGAccuggaucucccucgGCAGGUcCAUCGGuAUCg -3' miRNA: 3'- uGCCuCUGCU---------------UGUCCAuGUAGUC-UAG- -5' |
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14610 | 3' | -50.1 | NC_003521.1 | + | 18674 | 0.73 | 0.935311 |
Target: 5'- cACGGGGGCGcuGCAGGaccauCAUCAGAgcUCa -3' miRNA: 3'- -UGCCUCUGCu-UGUCCau---GUAGUCU--AG- -5' |
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14610 | 3' | -50.1 | NC_003521.1 | + | 17915 | 0.67 | 0.997734 |
Target: 5'- -gGGAGACGGACGGGcugUACGagguagccagcgaccUCAaGGUCu -3' miRNA: 3'- ugCCUCUGCUUGUCC---AUGU---------------AGU-CUAG- -5' |
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14610 | 3' | -50.1 | NC_003521.1 | + | 15316 | 0.68 | 0.99605 |
Target: 5'- gACGGcGcCGAGCGGGUG-AUCAGcgCg -3' miRNA: 3'- -UGCCuCuGCUUGUCCAUgUAGUCuaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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