Results 101 - 107 of 107 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14615 | 3' | -60.3 | NC_003521.1 | + | 225784 | 0.67 | 0.783598 |
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Target: 5'- cUCGCGCCGCUcgucaGGGUGCc-AGGcGUCCu -3' miRNA: 3'- -GGUGCGGCGA-----CCCGUGcuUCC-CAGGu -5' |
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| 14615 | 3' | -60.3 | NC_003521.1 | + | 118920 | 0.67 | 0.792182 |
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Target: 5'- uCgGCGCCGCggcgUGGGCGgCGGcgucccgucAGGG-CCAg -3' miRNA: 3'- -GgUGCGGCG----ACCCGU-GCU---------UCCCaGGU- -5' |
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| 14615 | 3' | -60.3 | NC_003521.1 | + | 218469 | 0.67 | 0.800639 |
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Target: 5'- aCCACGCCG-UGGGCGC--AGuGGcCCc -3' miRNA: 3'- -GGUGCGGCgACCCGUGcuUC-CCaGGu -5' |
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| 14615 | 3' | -60.3 | NC_003521.1 | + | 12203 | 0.67 | 0.800639 |
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Target: 5'- gCGCGCCGCgGGGuucuccucaCugGAGucGGGUUCGa -3' miRNA: 3'- gGUGCGGCGaCCC---------GugCUU--CCCAGGU- -5' |
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| 14615 | 3' | -60.3 | NC_003521.1 | + | 197165 | 0.67 | 0.807309 |
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Target: 5'- uCCGCGCUgggccugGCaGGGCugGAGGcgguggcGGUCCc -3' miRNA: 3'- -GGUGCGG-------CGaCCCGugCUUC-------CCAGGu -5' |
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| 14615 | 3' | -60.3 | NC_003521.1 | + | 92019 | 0.67 | 0.808962 |
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Target: 5'- -gGCGCgGCgGcGGCgGCGguGGGUCCGg -3' miRNA: 3'- ggUGCGgCGaC-CCG-UGCuuCCCAGGU- -5' |
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| 14615 | 3' | -60.3 | NC_003521.1 | + | 112789 | 0.66 | 0.862909 |
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Target: 5'- gCugGCCGCcuacGGGCACGcgcucuGGGacggCCGc -3' miRNA: 3'- gGugCGGCGa---CCCGUGCuu----CCCa---GGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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