miRNA: 3'- gCAUCUGCAUCuGCC--ACG--------GCACGaau -5'
miRNA: 3'- -GCAucUGCAUCUGC----CACGGCa-CGAau -5'
miRNA: 3'- gcauCUGCAUCugCCa--CGGCaCGAAu -5'
miRNA: 3'- -GCAUCUgCAUcu--------GCCACGGCACGaau -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14617 | 3' | -53.1 | NC_003521.1 | + | 84913 | 0.66 | 0.991955 |
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Target: 5'- aGUAGGCGUAGcCGGccUGCucgcagggCGUGCg-- -3' miRNA: 3'- gCAUCUGCAUCuGCC--ACG--------GCACGaau -5' |
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| 14617 | 3' | -53.1 | NC_003521.1 | + | 74404 | 0.66 | 0.991955 |
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Target: 5'- aCGU--GCGUGGACGaccuGUGCCGcgGCUa- -3' miRNA: 3'- -GCAucUGCAUCUGC----CACGGCa-CGAau -5' |
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| 14617 | 3' | -53.1 | NC_003521.1 | + | 147063 | 0.66 | 0.992971 |
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Target: 5'- ---cGACGUGGugGGagaGCCGgGCUUc -3' miRNA: 3'- gcauCUGCAUCugCCa--CGGCaCGAAu -5' |
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| 14617 | 3' | -53.1 | NC_003521.1 | + | 120687 | 0.66 | 0.992971 |
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Target: 5'- cCGUGGAgGUGagccgcaccuCGGUGCUGUGCc-- -3' miRNA: 3'- -GCAUCUgCAUcu--------GCCACGGCACGaau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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