Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1462 | 3' | -56 | NC_001335.1 | + | 9167 | 0.66 | 0.682 |
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Target: 5'- cCGCGU--CGGUCAUCGaccgaagCUCGGGCGUg -3' miRNA: 3'- -GCGUAccGCCAGUAGCa------GGGCUUGCA- -5' |
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| 1462 | 3' | -56 | NC_001335.1 | + | 35265 | 0.67 | 0.637427 |
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Target: 5'- gGCAUgaagaagGGCaccaaGGUCAUCGUCCagcgGGACGa -3' miRNA: 3'- gCGUA-------CCG-----CCAGUAGCAGGg---CUUGCa -5' |
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| 1462 | 3' | -56 | NC_001335.1 | + | 17040 | 0.67 | 0.627608 |
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Target: 5'- gGCuccGGUGaG-CAUCGUgCCGAGCGUg -3' miRNA: 3'- gCGua-CCGC-CaGUAGCAgGGCUUGCA- -5' |
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| 1462 | 3' | -56 | NC_001335.1 | + | 42106 | 0.67 | 0.616701 |
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Target: 5'- gGCGUGGgacuCGGUCGUCGgCaaGGACGa -3' miRNA: 3'- gCGUACC----GCCAGUAGCaGggCUUGCa -5' |
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| 1462 | 3' | -56 | NC_001335.1 | + | 3817 | 0.69 | 0.489205 |
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Target: 5'- uGCcUGGCuGGUCAcgUCGaCCgCGAACGUg -3' miRNA: 3'- gCGuACCG-CCAGU--AGCaGG-GCUUGCA- -5' |
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| 1462 | 3' | -56 | NC_001335.1 | + | 12882 | 1.08 | 0.001077 |
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Target: 5'- gCGCAUGGCGGUCAUCGUCCCGAACGUg -3' miRNA: 3'- -GCGUACCGCCAGUAGCAGGGCUUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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