Results 21 - 32 of 32 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14629 | 5' | -49.4 | NC_003521.1 | + | 58766 | 0.72 | 0.910463 |
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Target: 5'- aGGUCC-AGCCACUGcagGGCGGc- -3' miRNA: 3'- -CCAGGcUCGGUGAC---UCGCCaa -5' |
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| 14629 | 5' | -49.4 | NC_003521.1 | + | 125327 | 0.71 | 0.939096 |
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Target: 5'- gGGUCCG-GCCACaGAGCGa-- -3' miRNA: 3'- -CCAGGCuCGGUGaCUCGCcaa -5' |
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| 14629 | 5' | -49.4 | NC_003521.1 | + | 239348 | 0.71 | 0.944012 |
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Target: 5'- cGUCCGGGCguCUGcAGCGGg- -3' miRNA: 3'- cCAGGCUCGguGAC-UCGCCaa -5' |
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| 14629 | 5' | -49.4 | NC_003521.1 | + | 82473 | 0.71 | 0.953047 |
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Target: 5'- cGUCCG-GCCGCaGGGUGGUg -3' miRNA: 3'- cCAGGCuCGGUGaCUCGCCAa -5' |
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| 14629 | 5' | -49.4 | NC_003521.1 | + | 91569 | 0.69 | 0.981562 |
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Target: 5'- aGGUCCGc-CCACaagGGGCGGUg -3' miRNA: 3'- -CCAGGCucGGUGa--CUCGCCAa -5' |
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| 14629 | 5' | -49.4 | NC_003521.1 | + | 196391 | 0.68 | 0.985552 |
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Target: 5'- --gCCGAGCCGCcgucacgaUGGGCGGg- -3' miRNA: 3'- ccaGGCUCGGUG--------ACUCGCCaa -5' |
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| 14629 | 5' | -49.4 | NC_003521.1 | + | 121004 | 0.67 | 0.993374 |
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Target: 5'- aGUCgGAGCCGCUGcuccagaccuccucGGCGGc- -3' miRNA: 3'- cCAGgCUCGGUGAC--------------UCGCCaa -5' |
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| 14629 | 5' | -49.4 | NC_003521.1 | + | 177990 | 0.67 | 0.995349 |
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Target: 5'- --gCCGGGUCAgaGAGCGGg- -3' miRNA: 3'- ccaGGCUCGGUgaCUCGCCaa -5' |
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| 14629 | 5' | -49.4 | NC_003521.1 | + | 129230 | 0.66 | 0.996649 |
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Target: 5'- aGUCCGGcGCCGCUGGccacgcGCGGc- -3' miRNA: 3'- cCAGGCU-CGGUGACU------CGCCaa -5' |
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| 14629 | 5' | -49.4 | NC_003521.1 | + | 209522 | 0.66 | 0.997177 |
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Target: 5'- cGGUgaCCGucGCCGCUGccGCGGUUa -3' miRNA: 3'- -CCA--GGCu-CGGUGACu-CGCCAA- -5' |
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| 14629 | 5' | -49.4 | NC_003521.1 | + | 155441 | 0.66 | 0.997634 |
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Target: 5'- uGG-CCGAcCCGCUGGGCGaGUc -3' miRNA: 3'- -CCaGGCUcGGUGACUCGC-CAa -5' |
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| 14629 | 5' | -49.4 | NC_003521.1 | + | 227094 | 1.02 | 0.032326 |
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Target: 5'- cGGUCCGAGCCACUGAGCGGUUc -3' miRNA: 3'- -CCAGGCUCGGUGACUCGCCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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