Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 14633 | 3' | -64.2 | NC_003521.1 | + | 215030 | 0.66 | 0.672125 |
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Target: 5'- aGAGCCGUCUCguGaaaguGCGAca-GGGCCa -3' miRNA: 3'- -CUCGGCAGGGguCc----CGCUcagCCCGG- -5' |
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| 14633 | 3' | -64.2 | NC_003521.1 | + | 113341 | 0.66 | 0.681424 |
|
Target: 5'- -cGCCGgggggCuUCCAGGGgGA--CGGGCCg -3' miRNA: 3'- cuCGGCa----G-GGGUCCCgCUcaGCCCGG- -5' |
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| 14633 | 3' | -64.2 | NC_003521.1 | + | 135363 | 0.66 | 0.690689 |
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Target: 5'- -cGCCGUCUCCGcGGCG-GUuaucgcccgCGGGCg -3' miRNA: 3'- cuCGGCAGGGGUcCCGCuCA---------GCCCGg -5' |
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| 14633 | 3' | -64.2 | NC_003521.1 | + | 115655 | 0.66 | 0.699912 |
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Target: 5'- -uGUCGgcgCCCCAGuGGCGGGUgaagaagGcGGCCa -3' miRNA: 3'- cuCGGCa--GGGGUC-CCGCUCAg------C-CCGG- -5' |
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| 14633 | 3' | -64.2 | NC_003521.1 | + | 35018 | 0.66 | 0.699912 |
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Target: 5'- -cGUCGUgCCCGGcGGaCGcGGUUGGGCa -3' miRNA: 3'- cuCGGCAgGGGUC-CC-GC-UCAGCCCGg -5' |
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| 14633 | 3' | -64.2 | NC_003521.1 | + | 60690 | 0.66 | 0.644092 |
|
Target: 5'- cGGCCGcgcgggggaCCCgGGGGCGGcgCGGGUCg -3' miRNA: 3'- cUCGGCa--------GGGgUCCCGCUcaGCCCGG- -5' |
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| 14633 | 3' | -64.2 | NC_003521.1 | + | 39379 | 0.66 | 0.6628 |
|
Target: 5'- aGGCUGUCUgacgguaaggCGGGGCGAuGUUGGaGCCg -3' miRNA: 3'- cUCGGCAGGg---------GUCCCGCU-CAGCC-CGG- -5' |
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| 14633 | 3' | -64.2 | NC_003521.1 | + | 166065 | 0.66 | 0.644092 |
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Target: 5'- uAGCCagcauccacUCCCgCAGGGCcauagcggGAG-CGGGCCg -3' miRNA: 3'- cUCGGc--------AGGG-GUCCCG--------CUCaGCCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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