Results 21 - 31 of 31 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14634 | 5' | -43.3 | NC_003521.1 | + | 93638 | 0.73 | 0.999326 |
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Target: 5'- cGCCGccGCUAGUGGGGGGUCCGGg-- -3' miRNA: 3'- aUGGCa-UGAUUAUUUUUCGGGCUaaa -5' |
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| 14634 | 5' | -43.3 | NC_003521.1 | + | 87270 | 0.71 | 0.999949 |
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Target: 5'- cGCCGUGCgcGUGGccaacGGGCCCGAg-- -3' miRNA: 3'- aUGGCAUGauUAUUu----UUCGGGCUaaa -5' |
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| 14634 | 5' | -43.3 | NC_003521.1 | + | 84863 | 0.72 | 0.999879 |
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Target: 5'- cACCGUAC-GGUAGAAGgccGCCCGGUc- -3' miRNA: 3'- aUGGCAUGaUUAUUUUU---CGGGCUAaa -5' |
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| 14634 | 5' | -43.3 | NC_003521.1 | + | 79129 | 0.72 | 0.999842 |
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Target: 5'- cGCCGUACUcgcAGUAAAAGuagguacgcuGCCCGAa-- -3' miRNA: 3'- aUGGCAUGA---UUAUUUUU----------CGGGCUaaa -5' |
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| 14634 | 5' | -43.3 | NC_003521.1 | + | 75895 | 0.67 | 1 |
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Target: 5'- cGCCGgag----GAGGGGCCCGGUUg -3' miRNA: 3'- aUGGCaugauuaUUUUUCGGGCUAAa -5' |
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| 14634 | 5' | -43.3 | NC_003521.1 | + | 62225 | 0.72 | 0.999795 |
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Target: 5'- cGCCGUGCUGcagGAGGGcGCCCGGc-- -3' miRNA: 3'- aUGGCAUGAUua-UUUUU-CGGGCUaaa -5' |
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| 14634 | 5' | -43.3 | NC_003521.1 | + | 48064 | 0.67 | 1 |
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Target: 5'- gAUCGaGCUGAUGGAcGGCCCGc--- -3' miRNA: 3'- aUGGCaUGAUUAUUUuUCGGGCuaaa -5' |
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| 14634 | 5' | -43.3 | NC_003521.1 | + | 44245 | 0.66 | 1 |
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Target: 5'- cGCCGUGCgc---GGGAGUCCGGc-- -3' miRNA: 3'- aUGGCAUGauuauUUUUCGGGCUaaa -5' |
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| 14634 | 5' | -43.3 | NC_003521.1 | + | 39902 | 0.69 | 0.999997 |
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Target: 5'- gGCCG-ACUGcgGGAAGGCgCGAUg- -3' miRNA: 3'- aUGGCaUGAUuaUUUUUCGgGCUAaa -5' |
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| 14634 | 5' | -43.3 | NC_003521.1 | + | 39226 | 0.72 | 0.999879 |
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Target: 5'- aGCCGcgcaGCUGGUAAucgucGGAGCCCGAg-- -3' miRNA: 3'- aUGGCa---UGAUUAUU-----UUUCGGGCUaaa -5' |
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| 14634 | 5' | -43.3 | NC_003521.1 | + | 3144 | 0.72 | 0.999909 |
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Target: 5'- gUACCGUACUAcgAuaccacuGCCCGAc-- -3' miRNA: 3'- -AUGGCAUGAUuaUuuuu---CGGGCUaaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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