Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1464 | 5' | -54.5 | NC_001335.1 | + | 31799 | 0.66 | 0.691153 |
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Target: 5'- cGCGGcaagcCCcGGUcAGCCAGCCGGUUg- -3' miRNA: 3'- -UGCCaa---GGuCCA-UCGGUUGGUCAGac -5' |
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| 1464 | 5' | -54.5 | NC_001335.1 | + | 47154 | 0.67 | 0.668969 |
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Target: 5'- aGCGGaUCU-GGUcgacguggaugcAGCCGACgCGGUCUGg -3' miRNA: 3'- -UGCCaAGGuCCA------------UCGGUUG-GUCAGAC- -5' |
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| 1464 | 5' | -54.5 | NC_001335.1 | + | 11491 | 0.67 | 0.64776 |
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Target: 5'- uCGGgcUCgAGGUAGCCcucgaacaucgagucGCCGGUCUGc -3' miRNA: 3'- uGCCa-AGgUCCAUCGGu--------------UGGUCAGAC- -5' |
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| 1464 | 5' | -54.5 | NC_001335.1 | + | 11756 | 0.67 | 0.646642 |
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Target: 5'- cAUGGUgcCCAGGUAGUUGGCCGGg--- -3' miRNA: 3'- -UGCCAa-GGUCCAUCGGUUGGUCagac -5' |
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| 1464 | 5' | -54.5 | NC_001335.1 | + | 31263 | 0.68 | 0.624259 |
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Target: 5'- cUGGUUCUcGaUGGCCAACCGGUUg- -3' miRNA: 3'- uGCCAAGGuCcAUCGGUUGGUCAGac -5' |
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| 1464 | 5' | -54.5 | NC_001335.1 | + | 30472 | 0.68 | 0.613073 |
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Target: 5'- gGCGGaaUCCAGGUGGCUgaGGCCA-UCUc -3' miRNA: 3'- -UGCCa-AGGUCCAUCGG--UUGGUcAGAc -5' |
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| 1464 | 5' | -54.5 | NC_001335.1 | + | 36836 | 0.73 | 0.324907 |
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Target: 5'- gGCGGUUCCuGG-AGCCGcugcGCCAgGUCUa -3' miRNA: 3'- -UGCCAAGGuCCaUCGGU----UGGU-CAGAc -5' |
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| 1464 | 5' | -54.5 | NC_001335.1 | + | 1516 | 0.74 | 0.272056 |
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Target: 5'- gUGGUgaUCCGGGUccaaAGCCuGCCAGUCUc -3' miRNA: 3'- uGCCA--AGGUCCA----UCGGuUGGUCAGAc -5' |
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| 1464 | 5' | -54.5 | NC_001335.1 | + | 13235 | 1.09 | 0.001041 |
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Target: 5'- cACGGUUCCAGGUAGCCAACCAGUCUGg -3' miRNA: 3'- -UGCCAAGGUCCAUCGGUUGGUCAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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