Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14643 | 3' | -52.1 | NC_003521.1 | + | 187689 | 0.68 | 0.976609 |
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Target: 5'- -gCGCCCUcgGgcGACUGCGUGUaccugaUCUCg -3' miRNA: 3'- caGUGGGGa-CuuUUGGCGCACA------AGAG- -5' |
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| 14643 | 3' | -52.1 | NC_003521.1 | + | 216221 | 0.7 | 0.950469 |
|
Target: 5'- cUCGCCCCUGAGGugaCGCGU--UCUa -3' miRNA: 3'- cAGUGGGGACUUUug-GCGCAcaAGAg -5' |
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| 14643 | 3' | -52.1 | NC_003521.1 | + | 223159 | 0.73 | 0.868531 |
|
Target: 5'- -aCGCCCgcaguCUGGAGGCCGCaucgcucaucacgGUGUUCUCu -3' miRNA: 3'- caGUGGG-----GACUUUUGGCG-------------CACAAGAG- -5' |
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| 14643 | 3' | -52.1 | NC_003521.1 | + | 235233 | 0.69 | 0.961883 |
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Target: 5'- -gCACCUCU----ACCGCGUGUUCg- -3' miRNA: 3'- caGUGGGGAcuuuUGGCGCACAAGag -5' |
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| 14643 | 3' | -52.1 | NC_003521.1 | + | 239302 | 0.67 | 0.986167 |
|
Target: 5'- -cCAuCCCCUGAGGcguucguggucguuGCUGUGUG-UCUCa -3' miRNA: 3'- caGU-GGGGACUUU--------------UGGCGCACaAGAG- -5' |
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| 14643 | 3' | -52.1 | NC_003521.1 | + | 240324 | 1.1 | 0.008858 |
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Target: 5'- cGUCACCCCUGAAAACCGCGUGUUCUCu -3' miRNA: 3'- -CAGUGGGGACUUUUGGCGCACAAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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