Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14643 | 5' | -49.7 | NC_003521.1 | + | 216908 | 0.7 | 0.987076 |
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Target: 5'- aGGAGgGCGcGGUggcagCGGAGGCGGu -3' miRNA: 3'- gCCUCgUGCaUCAaaa--GUCUCCGUUu -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 127780 | 0.7 | 0.97931 |
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Target: 5'- uGGAGCACGgcgcGUUUgugaaAGAGGCGc- -3' miRNA: 3'- gCCUCGUGCau--CAAAag---UCUCCGUuu -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 112198 | 0.71 | 0.96852 |
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Target: 5'- aGGGGCGCGUGuccgaagCGGAGGCGc- -3' miRNA: 3'- gCCUCGUGCAUcaaaa--GUCUCCGUuu -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 198640 | 0.73 | 0.933033 |
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Target: 5'- gGGAaacgccgugcugucuGCACGUGGUUUUCGGAcGCGAu -3' miRNA: 3'- gCCU---------------CGUGCAUCAAAAGUCUcCGUUu -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 57219 | 0.74 | 0.894693 |
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Target: 5'- uCGGAG-GCGUAGc--UCAGAGGCGAGc -3' miRNA: 3'- -GCCUCgUGCAUCaaaAGUCUCCGUUU- -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 150516 | 0.77 | 0.788028 |
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Target: 5'- aGGGGUACGUAGUUUUCuuaccGGGGUAc- -3' miRNA: 3'- gCCUCGUGCAUCAAAAGu----CUCCGUuu -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 133216 | 0.78 | 0.749281 |
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Target: 5'- uCGGGGCGCGgg---UUCAGGGGCGAu -3' miRNA: 3'- -GCCUCGUGCaucaaAAGUCUCCGUUu -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 40132 | 1.07 | 0.022154 |
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Target: 5'- gCGGAGCACGUAGUUUUCAGAGGCAAAc -3' miRNA: 3'- -GCCUCGUGCAUCAAAAGUCUCCGUUU- -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 240360 | 1.07 | 0.022154 |
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Target: 5'- gCGGAGCACGUAGUUUUCAGAGGCAAAc -3' miRNA: 3'- -GCCUCGUGCAUCAAAAGUCUCCGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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