Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14643 | 5' | -49.7 | NC_003521.1 | + | 118684 | 0.68 | 0.995781 |
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Target: 5'- uCGGGGCACGUGcugaaagccGUgUUCAGccgcGGCGAGa -3' miRNA: 3'- -GCCUCGUGCAU---------CAaAAGUCu---CCGUUU- -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 494 | 0.68 | 0.99428 |
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Target: 5'- cCGaGAGaaCACGcGGUUUUCAGGGGUGAc -3' miRNA: 3'- -GC-CUC--GUGCaUCAAAAGUCUCCGUUu -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 180398 | 0.69 | 0.993382 |
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Target: 5'- uGGGGC-UGUAGacg-UAGAGGCAGAg -3' miRNA: 3'- gCCUCGuGCAUCaaaaGUCUCCGUUU- -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 216908 | 0.7 | 0.987076 |
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Target: 5'- aGGAGgGCGcGGUggcagCGGAGGCGGu -3' miRNA: 3'- gCCUCgUGCaUCAaaa--GUCUCCGUUu -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 127780 | 0.7 | 0.97931 |
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Target: 5'- uGGAGCACGgcgcGUUUgugaaAGAGGCGc- -3' miRNA: 3'- gCCUCGUGCau--CAAAag---UCUCCGUuu -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 198640 | 0.73 | 0.933033 |
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Target: 5'- gGGAaacgccgugcugucuGCACGUGGUUUUCGGAcGCGAu -3' miRNA: 3'- gCCU---------------CGUGCAUCAAAAGUCUcCGUUu -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 150516 | 0.77 | 0.788028 |
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Target: 5'- aGGGGUACGUAGUUUUCuuaccGGGGUAc- -3' miRNA: 3'- gCCUCGUGCAUCAAAAGu----CUCCGUuu -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 40132 | 1.07 | 0.022154 |
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Target: 5'- gCGGAGCACGUAGUUUUCAGAGGCAAAc -3' miRNA: 3'- -GCCUCGUGCAUCAAAAGUCUCCGUUU- -5' |
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| 14643 | 5' | -49.7 | NC_003521.1 | + | 75555 | 0.66 | 0.999443 |
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Target: 5'- aCGaGAGCGagacCGUGGUgugcgugUUCuGAGGCGAc -3' miRNA: 3'- -GC-CUCGU----GCAUCAa------AAGuCUCCGUUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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