Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14649 | 3' | -54.5 | NC_003525.1 | + | 45096 | 0.66 | 0.765048 |
Target: 5'- -cAUCuuUGCCagaCCGCGcugGGUAAGCGUu -3' miRNA: 3'- aaUAGcgACGGg--GGCGC---UUAUUCGCG- -5' |
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14649 | 3' | -54.5 | NC_003525.1 | + | 59417 | 0.66 | 0.762987 |
Target: 5'- gUUAUCGCgguaaugauucaGCCCCa--GAcUAAGCGCa -3' miRNA: 3'- -AAUAGCGa-----------CGGGGgcgCUuAUUCGCG- -5' |
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14649 | 3' | -54.5 | NC_003525.1 | + | 43457 | 0.67 | 0.679245 |
Target: 5'- -gAUUGCUGCUUCUuCGGAUGAGUGa -3' miRNA: 3'- aaUAGCGACGGGGGcGCUUAUUCGCg -5' |
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14649 | 3' | -54.5 | NC_003525.1 | + | 7358 | 0.7 | 0.53578 |
Target: 5'- --uUCGCUgGCCCCUGCagGAAUu-GUGCg -3' miRNA: 3'- aauAGCGA-CGGGGGCG--CUUAuuCGCG- -5' |
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14649 | 3' | -54.5 | NC_003525.1 | + | 58883 | 0.72 | 0.423166 |
Target: 5'- uUUGUCGUUaaUCCCCGuCGAAUGAGUGUg -3' miRNA: 3'- -AAUAGCGAc-GGGGGC-GCUUAUUCGCG- -5' |
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14649 | 3' | -54.5 | NC_003525.1 | + | 3322 | 0.73 | 0.359311 |
Target: 5'- ---aCGCUGCCCCUGUGucaGGGCGa -3' miRNA: 3'- aauaGCGACGGGGGCGCuuaUUCGCg -5' |
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14649 | 3' | -54.5 | NC_003525.1 | + | 56160 | 0.8 | 0.120867 |
Target: 5'- cUGUCGaugaUGCCCCCGCGuuu-AGCGCc -3' miRNA: 3'- aAUAGCg---ACGGGGGCGCuuauUCGCG- -5' |
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14649 | 3' | -54.5 | NC_003525.1 | + | 3558 | 1.1 | 0.000988 |
Target: 5'- cUUAUCGCUGCCCCCGCGAAUAAGCGCa -3' miRNA: 3'- -AAUAGCGACGGGGGCGCUUAUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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