Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1465 | 5' | -51.6 | NC_001335.1 | + | 14094 | 0.66 | 0.860544 |
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Target: 5'- gACCGUCUCugguguAGGcGGUCAUuucgaccaccGGCa -3' miRNA: 3'- -UGGCAGAGuau---UCCuCCAGUGua--------CCG- -5' |
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| 1465 | 5' | -51.6 | NC_001335.1 | + | 4717 | 0.66 | 0.860544 |
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Target: 5'- gACCGgggCUUGguauGGAGGUCGCAccgacUGGg -3' miRNA: 3'- -UGGCa--GAGUauu-CCUCCAGUGU-----ACCg -5' |
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| 1465 | 5' | -51.6 | NC_001335.1 | + | 14880 | 0.67 | 0.851943 |
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Target: 5'- gGCUGUCUaccccggagGGGGAGGUUuccuUGGCg -3' miRNA: 3'- -UGGCAGAgua------UUCCUCCAGugu-ACCG- -5' |
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| 1465 | 5' | -51.6 | NC_001335.1 | + | 40961 | 0.68 | 0.79554 |
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Target: 5'- cGCCGUCgUUGUGcGGGccGGUgACGUGGUg -3' miRNA: 3'- -UGGCAG-AGUAUuCCU--CCAgUGUACCG- -5' |
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| 1465 | 5' | -51.6 | NC_001335.1 | + | 38823 | 0.74 | 0.45564 |
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Target: 5'- aGCUGUCUCGaAcuugacauccaccAGGAGGaaACAUGGCa -3' miRNA: 3'- -UGGCAGAGUaU-------------UCCUCCagUGUACCG- -5' |
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| 1465 | 5' | -51.6 | NC_001335.1 | + | 13805 | 1.13 | 0.001199 |
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Target: 5'- cACCGUCUCAUAAGGAGGUCACAUGGCg -3' miRNA: 3'- -UGGCAGAGUAUUCCUCCAGUGUACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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