Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14654 | 5' | -52.5 | NC_003525.1 | + | 18863 | 0.66 | 0.874087 |
Target: 5'- --aUGCGGuugugaccaGUCGCAgcauaauauucaUCCACCGCAgCCu -3' miRNA: 3'- cugACGCC---------UAGUGU------------AGGUGGUGUaGG- -5' |
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14654 | 5' | -52.5 | NC_003525.1 | + | 25525 | 0.66 | 0.874087 |
Target: 5'- cGAUgGCGGAUCAgCgAUUCGCuCAgGUCCu -3' miRNA: 3'- -CUGaCGCCUAGU-G-UAGGUG-GUgUAGG- -5' |
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14654 | 5' | -52.5 | NC_003525.1 | + | 20091 | 0.66 | 0.857788 |
Target: 5'- uGCUGCGGGUCGgCGUCaauggACaaaaACGUCUg -3' miRNA: 3'- cUGACGCCUAGU-GUAGg----UGg---UGUAGG- -5' |
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14654 | 5' | -52.5 | NC_003525.1 | + | 32341 | 0.67 | 0.849273 |
Target: 5'- --gUGCGGcaauuucaccguGUCGCAUCCcuguaaaaacaGCCACuGUCCa -3' miRNA: 3'- cugACGCC------------UAGUGUAGG-----------UGGUG-UAGG- -5' |
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14654 | 5' | -52.5 | NC_003525.1 | + | 43449 | 0.67 | 0.831552 |
Target: 5'- uACUGauGccauuUCAUAUCCuuCCGCAUCCa -3' miRNA: 3'- cUGACgcCu----AGUGUAGGu-GGUGUAGG- -5' |
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14654 | 5' | -52.5 | NC_003525.1 | + | 21655 | 0.67 | 0.822364 |
Target: 5'- --gUGCuG-UCGCGUUCugCGCAUCCg -3' miRNA: 3'- cugACGcCuAGUGUAGGugGUGUAGG- -5' |
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14654 | 5' | -52.5 | NC_003525.1 | + | 13757 | 0.68 | 0.803386 |
Target: 5'- cAUUGCGGAUCACcaGUCC--UACAUUCu -3' miRNA: 3'- cUGACGCCUAGUG--UAGGugGUGUAGG- -5' |
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14654 | 5' | -52.5 | NC_003525.1 | + | 24582 | 0.68 | 0.801446 |
Target: 5'- cGCUGacaccacCGGAUCACcauuuuuAUCCACgGCAUCa -3' miRNA: 3'- cUGAC-------GCCUAGUG-------UAGGUGgUGUAGg -5' |
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14654 | 5' | -52.5 | NC_003525.1 | + | 46064 | 0.68 | 0.783673 |
Target: 5'- ---cGCGGugGUUACGUCCGUCACGUUCa -3' miRNA: 3'- cugaCGCC--UAGUGUAGGUGGUGUAGG- -5' |
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14654 | 5' | -52.5 | NC_003525.1 | + | 20490 | 0.69 | 0.742409 |
Target: 5'- -uCUGCGGccUgACGUCUGCCACGauaUCCg -3' miRNA: 3'- cuGACGCCu-AgUGUAGGUGGUGU---AGG- -5' |
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14654 | 5' | -52.5 | NC_003525.1 | + | 21515 | 0.7 | 0.644037 |
Target: 5'- -gUUGUGGuaaaACGaCCACCACGUCCg -3' miRNA: 3'- cuGACGCCuag-UGUaGGUGGUGUAGG- -5' |
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14654 | 5' | -52.5 | NC_003525.1 | + | 5011 | 0.73 | 0.512432 |
Target: 5'- cGCUGCuGAcucaUACGUCCGCCuGCAUCCa -3' miRNA: 3'- cUGACGcCUa---GUGUAGGUGG-UGUAGG- -5' |
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14654 | 5' | -52.5 | NC_003525.1 | + | 20665 | 0.73 | 0.470812 |
Target: 5'- aGCUGCGGAugcgUCACccUCUguuGCCAUAUCCg -3' miRNA: 3'- cUGACGCCU----AGUGu-AGG---UGGUGUAGG- -5' |
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14654 | 5' | -52.5 | NC_003525.1 | + | 59545 | 0.75 | 0.411817 |
Target: 5'- -cCUGCGGAUCAacgCCACC-UGUCCa -3' miRNA: 3'- cuGACGCCUAGUguaGGUGGuGUAGG- -5' |
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14654 | 5' | -52.5 | NC_003525.1 | + | 11169 | 1.13 | 0.001209 |
Target: 5'- gGACUGCGGAUCACAUCCACCACAUCCg -3' miRNA: 3'- -CUGACGCCUAGUGUAGGUGGUGUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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