miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
14656 5' -51.7 NC_003525.1 + 37662 0.66 0.903764
Target:  5'- -aUAAUGcaGGCCUUCACGCUucaugcgcgGGUCa -3'
miRNA:   3'- caGUUACuaCCGGAAGUGCGG---------CUAGa -5'
14656 5' -51.7 NC_003525.1 + 49134 0.66 0.895986
Target:  5'- uGUCGGUGAUGcuucuuugcugucGCC-UCAUGCCGcccuUCUg -3'
miRNA:   3'- -CAGUUACUAC-------------CGGaAGUGCGGCu---AGA- -5'
14656 5' -51.7 NC_003525.1 + 56660 0.67 0.865731
Target:  5'- gGUCAG-GAUGGCacaccgUUCACGCUGAc-- -3'
miRNA:   3'- -CAGUUaCUACCGg-----AAGUGCGGCUaga -5'
14656 5' -51.7 NC_003525.1 + 13067 1.08 0.002648
Target:  5'- gGUCAAUGAUGGCCUUCACGCCGAUCUg -3'
miRNA:   3'- -CAGUUACUACCGGAAGUGCGGCUAGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.