Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14657 | 5' | -56.3 | NC_003525.1 | + | 48115 | 0.66 | 0.684819 |
Target: 5'- uUCAGCCGccgCAGCaacuGAu-GGUGCCCAUg -3' miRNA: 3'- -GGUCGGUa--GUCGg---CUuuCCGUGGGUG- -5' |
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14657 | 5' | -56.3 | NC_003525.1 | + | 18464 | 0.66 | 0.673993 |
Target: 5'- aCCAGCg--CAGCagGAAGGGUACgUACu -3' miRNA: 3'- -GGUCGguaGUCGg-CUUUCCGUGgGUG- -5' |
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14657 | 5' | -56.3 | NC_003525.1 | + | 26797 | 0.66 | 0.673993 |
Target: 5'- aCCGGCag-CAcgggacGCCGucAGGuCACCCGCc -3' miRNA: 3'- -GGUCGguaGU------CGGCuuUCC-GUGGGUG- -5' |
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14657 | 5' | -56.3 | NC_003525.1 | + | 49295 | 0.66 | 0.652233 |
Target: 5'- -aGGCCAaaGGCgGuAAGGCACgCGCg -3' miRNA: 3'- ggUCGGUagUCGgCuUUCCGUGgGUG- -5' |
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14657 | 5' | -56.3 | NC_003525.1 | + | 1879 | 0.66 | 0.651142 |
Target: 5'- uCCAGCggcgacgCAUCAGCC-AucGGCAgUCACu -3' miRNA: 3'- -GGUCG-------GUAGUCGGcUuuCCGUgGGUG- -5' |
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14657 | 5' | -56.3 | NC_003525.1 | + | 55062 | 0.66 | 0.641321 |
Target: 5'- aCCAGCUgucgaaAUCAGCgCacuaccauAAGGCugCCACc -3' miRNA: 3'- -GGUCGG------UAGUCG-Gcu------UUCCGugGGUG- -5' |
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14657 | 5' | -56.3 | NC_003525.1 | + | 50859 | 0.67 | 0.616207 |
Target: 5'- uUCAGCCuucuggucuucaagGUCGGCCGcAAGGCGCa--- -3' miRNA: 3'- -GGUCGG--------------UAGUCGGCuUUCCGUGggug -5' |
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14657 | 5' | -56.3 | NC_003525.1 | + | 20599 | 0.67 | 0.597685 |
Target: 5'- gCAGCuCAUC-GCCGGGAaGCugCCAg -3' miRNA: 3'- gGUCG-GUAGuCGGCUUUcCGugGGUg -5' |
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14657 | 5' | -56.3 | NC_003525.1 | + | 19715 | 0.67 | 0.586826 |
Target: 5'- uUCGGUCAUCGGCguauucagcgcaCGAAcuGCACCCGg -3' miRNA: 3'- -GGUCGGUAGUCG------------GCUUucCGUGGGUg -5' |
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14657 | 5' | -56.3 | NC_003525.1 | + | 57880 | 0.71 | 0.395187 |
Target: 5'- uCUGGCCAUCuGCCGuGGAGcCACCCGg -3' miRNA: 3'- -GGUCGGUAGuCGGC-UUUCcGUGGGUg -5' |
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14657 | 5' | -56.3 | NC_003525.1 | + | 15895 | 0.71 | 0.372168 |
Target: 5'- cCCcGUCAacugguugcugagguUCAGCCGAuauGGCACCCGu -3' miRNA: 3'- -GGuCGGU---------------AGUCGGCUuu-CCGUGGGUg -5' |
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14657 | 5' | -56.3 | NC_003525.1 | + | 9709 | 0.73 | 0.3118 |
Target: 5'- aCCGGaCC-UCaggGGCCGAAGGGCgACCCGg -3' miRNA: 3'- -GGUC-GGuAG---UCGGCUUUCCG-UGGGUg -5' |
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14657 | 5' | -56.3 | NC_003525.1 | + | 57084 | 0.74 | 0.24247 |
Target: 5'- gCCAGUCAgUCAGCCGGAAGcaaACCCGa -3' miRNA: 3'- -GGUCGGU-AGUCGGCUUUCcg-UGGGUg -5' |
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14657 | 5' | -56.3 | NC_003525.1 | + | 54578 | 0.76 | 0.186462 |
Target: 5'- aCCAgGCCGaacUCA-CCGGGAGGCACCCGg -3' miRNA: 3'- -GGU-CGGU---AGUcGGCUUUCCGUGGGUg -5' |
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14657 | 5' | -56.3 | NC_003525.1 | + | 15666 | 1.12 | 0.000544 |
Target: 5'- uCCAGCCAUCAGCCGAAAGGCACCCACg -3' miRNA: 3'- -GGUCGGUAGUCGGCUUUCCGUGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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