Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14660 | 3' | -53.4 | NC_003525.1 | + | 2709 | 0.66 | 0.834495 |
Target: 5'- cCACCAggUUACUGAUAGCCacGCCuGa -3' miRNA: 3'- cGUGGU--AGUGACUGUCGGaaUGGcCa -5' |
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14660 | 3' | -53.4 | NC_003525.1 | + | 48027 | 0.67 | 0.796871 |
Target: 5'- gGCACCAUCaguuGCUGcgGCGGCUgaaaaauggaACCGGa -3' miRNA: 3'- -CGUGGUAG----UGAC--UGUCGGaa--------UGGCCa -5' |
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14660 | 3' | -53.4 | NC_003525.1 | + | 56598 | 0.67 | 0.786999 |
Target: 5'- gGCAUa--CGCUGGCuuuccGCUUUGCCGGg -3' miRNA: 3'- -CGUGguaGUGACUGu----CGGAAUGGCCa -5' |
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14660 | 3' | -53.4 | NC_003525.1 | + | 8007 | 0.67 | 0.776962 |
Target: 5'- uUACCcugacggCGCUGACAGUUUcACCGGa -3' miRNA: 3'- cGUGGua-----GUGACUGUCGGAaUGGCCa -5' |
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14660 | 3' | -53.4 | NC_003525.1 | + | 51288 | 0.67 | 0.776962 |
Target: 5'- aGCACCAcCACUGAUuugAGaa-UGCCGGg -3' miRNA: 3'- -CGUGGUaGUGACUG---UCggaAUGGCCa -5' |
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14660 | 3' | -53.4 | NC_003525.1 | + | 47073 | 0.68 | 0.735403 |
Target: 5'- cGUGCCAUUuuaGCUG-CGGCCUgauuuaUGCUGGUu -3' miRNA: 3'- -CGUGGUAG---UGACuGUCGGA------AUGGCCA- -5' |
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14660 | 3' | -53.4 | NC_003525.1 | + | 56672 | 0.68 | 0.707434 |
Target: 5'- aCACCGUucaCGCUGACGcagucaccggucaguGCCUUugCGGc -3' miRNA: 3'- cGUGGUA---GUGACUGU---------------CGGAAugGCCa -5' |
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14660 | 3' | -53.4 | NC_003525.1 | + | 207 | 0.69 | 0.681159 |
Target: 5'- uGCAuUCAUCAgaGACGGCauuaagcgUACCGGUu -3' miRNA: 3'- -CGU-GGUAGUgaCUGUCGga------AUGGCCA- -5' |
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14660 | 3' | -53.4 | NC_003525.1 | + | 12493 | 0.69 | 0.636833 |
Target: 5'- aUACCAUCAcCUGcCAGUUUUGcCCGGUu -3' miRNA: 3'- cGUGGUAGU-GACuGUCGGAAU-GGCCA- -5' |
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14660 | 3' | -53.4 | NC_003525.1 | + | 22444 | 0.7 | 0.624603 |
Target: 5'- aGCACCGUCACcggaUGACAaaauccGCUuugacguUUACCGGa -3' miRNA: 3'- -CGUGGUAGUG----ACUGU------CGG-------AAUGGCCa -5' |
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14660 | 3' | -53.4 | NC_003525.1 | + | 9751 | 0.72 | 0.50262 |
Target: 5'- cGCACCuguuucuccuuucgGUCcCUGugGGCC-UGCCGGg -3' miRNA: 3'- -CGUGG--------------UAGuGACugUCGGaAUGGCCa -5' |
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14660 | 3' | -53.4 | NC_003525.1 | + | 5453 | 0.73 | 0.425001 |
Target: 5'- -aACCAUCAuCUGGuucCAGuCCUUACCGGa -3' miRNA: 3'- cgUGGUAGU-GACU---GUC-GGAAUGGCCa -5' |
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14660 | 3' | -53.4 | NC_003525.1 | + | 19640 | 1.09 | 0.00172 |
Target: 5'- gGCACCAUCACUGACAGCCUUACCGGUu -3' miRNA: 3'- -CGUGGUAGUGACUGUCGGAAUGGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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