Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14661 | 3' | -56.2 | NC_003525.1 | + | 26335 | 0.66 | 0.62177 |
Target: 5'- cGCCGCCugcUGGCGUg-ACAaaCCGGCAu -3' miRNA: 3'- -CGGCGGu--ACCGUAagUGUc-GGUCGUu -5' |
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14661 | 3' | -56.2 | NC_003525.1 | + | 58645 | 0.66 | 0.599539 |
Target: 5'- gGCUgGCC-UGGCAaUCACAGUUGGCu- -3' miRNA: 3'- -CGG-CGGuACCGUaAGUGUCGGUCGuu -5' |
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14661 | 3' | -56.2 | NC_003525.1 | + | 59524 | 0.66 | 0.599539 |
Target: 5'- cGuuGCCGcUGGUuacCGCAGCCuGCGGa -3' miRNA: 3'- -CggCGGU-ACCGuaaGUGUCGGuCGUU- -5' |
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14661 | 3' | -56.2 | NC_003525.1 | + | 17712 | 0.67 | 0.588462 |
Target: 5'- cGCCGCCAUcuGUuuGUUCGCAGCCu-CAAu -3' miRNA: 3'- -CGGCGGUAc-CG--UAAGUGUCGGucGUU- -5' |
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14661 | 3' | -56.2 | NC_003525.1 | + | 35913 | 0.67 | 0.588462 |
Target: 5'- aGCgCGCCGcUGGCAUUCGCAucaaaagagGUgAGUAAu -3' miRNA: 3'- -CG-GCGGU-ACCGUAAGUGU---------CGgUCGUU- -5' |
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14661 | 3' | -56.2 | NC_003525.1 | + | 48249 | 0.68 | 0.491418 |
Target: 5'- aGgUGCUAUGGCAUggUCaACGGCUGGCGc -3' miRNA: 3'- -CgGCGGUACCGUA--AG-UGUCGGUCGUu -5' |
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14661 | 3' | -56.2 | NC_003525.1 | + | 35787 | 0.68 | 0.491418 |
Target: 5'- cGCCGCCAUaccGGUAUU-GCAGCggcacgacgagCAGCAAa -3' miRNA: 3'- -CGGCGGUA---CCGUAAgUGUCG-----------GUCGUU- -5' |
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14661 | 3' | -56.2 | NC_003525.1 | + | 38972 | 0.69 | 0.450654 |
Target: 5'- uUCGCCAgacGGCAUUUAaaggugauGCCAGCGAu -3' miRNA: 3'- cGGCGGUa--CCGUAAGUgu------CGGUCGUU- -5' |
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14661 | 3' | -56.2 | NC_003525.1 | + | 3183 | 0.69 | 0.440754 |
Target: 5'- cGCCGCCAgGGCAcaUGCAGCU-GCAc -3' miRNA: 3'- -CGGCGGUaCCGUaaGUGUCGGuCGUu -5' |
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14661 | 3' | -56.2 | NC_003525.1 | + | 56478 | 0.71 | 0.332461 |
Target: 5'- -aCGCCAuaacauacUGGU--UCGCAGCCAGCGc -3' miRNA: 3'- cgGCGGU--------ACCGuaAGUGUCGGUCGUu -5' |
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14661 | 3' | -56.2 | NC_003525.1 | + | 6821 | 0.8 | 0.0827 |
Target: 5'- gGCUGCCGUGGCAUUCAgCcGCCAGUc- -3' miRNA: 3'- -CGGCGGUACCGUAAGU-GuCGGUCGuu -5' |
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14661 | 3' | -56.2 | NC_003525.1 | + | 20206 | 1.08 | 0.000841 |
Target: 5'- uGCCGCCAUGGCAUUCACAGCCAGCAAu -3' miRNA: 3'- -CGGCGGUACCGUAAGUGUCGGUCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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