Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14666 | 3' | -57.5 | NC_003525.1 | + | 6218 | 0.67 | 0.54301 |
Target: 5'- cCCCGUCACGCUGUGu---GGUGuacGGCu -3' miRNA: 3'- uGGGCGGUGCGACGCcaauUCGC---UCG- -5' |
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14666 | 3' | -57.5 | NC_003525.1 | + | 59615 | 0.68 | 0.522144 |
Target: 5'- gAUCCG-CAgGCUGCGGUaaccAGCG-GCa -3' miRNA: 3'- -UGGGCgGUgCGACGCCAau--UCGCuCG- -5' |
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14666 | 3' | -57.5 | NC_003525.1 | + | 1631 | 0.68 | 0.511827 |
Target: 5'- aGCCCGCUcugAUGCcuaUGCGGaacaaaucaAGGCGGGCc -3' miRNA: 3'- -UGGGCGG---UGCG---ACGCCaa-------UUCGCUCG- -5' |
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14666 | 3' | -57.5 | NC_003525.1 | + | 52808 | 0.7 | 0.387132 |
Target: 5'- cGCCCgGCCcuuuaGCUcaGUGGUgaGAGCGAGCg -3' miRNA: 3'- -UGGG-CGGug---CGA--CGCCAa-UUCGCUCG- -5' |
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14666 | 3' | -57.5 | NC_003525.1 | + | 26591 | 1.1 | 0.000572 |
Target: 5'- cACCCGCCACGCUGCGGUUAAGCGAGCc -3' miRNA: 3'- -UGGGCGGUGCGACGCCAAUUCGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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