Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14666 | 5' | -57.4 | NC_003525.1 | + | 8807 | 0.66 | 0.566246 |
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Target: 5'- gGGCCUGCUggUCCGC-GUGGUgagAAGgGCg -3' miRNA: 3'- aUUGGACGA--GGGUGuCGCCG---UUCgCG- -5' |
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| 14666 | 5' | -57.4 | NC_003525.1 | + | 47 | 0.66 | 0.55548 |
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Target: 5'- -cGCCUGCauaUCUCAgAGCacGGCAAaauuuGCGCg -3' miRNA: 3'- auUGGACG---AGGGUgUCG--CCGUU-----CGCG- -5' |
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| 14666 | 5' | -57.4 | NC_003525.1 | + | 56457 | 0.67 | 0.49239 |
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Target: 5'- cAGCCagcGCUCCCGaaaccuuaaAGCGGCA-GCGa -3' miRNA: 3'- aUUGGa--CGAGGGUg--------UCGCCGUuCGCg -5' |
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| 14666 | 5' | -57.4 | NC_003525.1 | + | 7018 | 0.69 | 0.386496 |
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Target: 5'- -uGCCUGUUCUCcCGGUgaGGUGAGCGUg -3' miRNA: 3'- auUGGACGAGGGuGUCG--CCGUUCGCG- -5' |
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| 14666 | 5' | -57.4 | NC_003525.1 | + | 38960 | 0.71 | 0.31952 |
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Target: 5'- cAGCCaGCuUCCCAgcCAGCGuuGCGAGUGCu -3' miRNA: 3'- aUUGGaCG-AGGGU--GUCGC--CGUUCGCG- -5' |
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| 14666 | 5' | -57.4 | NC_003525.1 | + | 11688 | 0.71 | 0.303445 |
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Target: 5'- aGGCCUGUcauauucUCUgGCGGCGGCAGGUa- -3' miRNA: 3'- aUUGGACG-------AGGgUGUCGCCGUUCGcg -5' |
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| 14666 | 5' | -57.4 | NC_003525.1 | + | 26557 | 1.1 | 0.000432 |
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Target: 5'- aUAACCUGCUCCCACAGCGGCAAGCGCg -3' miRNA: 3'- -AUUGGACGAGGGUGUCGCCGUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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