Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1467 | 5' | -60.3 | NC_001335.1 | + | 1156 | 0.67 | 0.350572 |
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Target: 5'- gAUCGGGCggcguaggCGGCGUCgG-AGGCgGGGg -3' miRNA: 3'- -UAGUCCGa-------GCCGCAGgCgUCUGgCUC- -5' |
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| 1467 | 5' | -60.3 | NC_001335.1 | + | 9831 | 0.68 | 0.318894 |
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Target: 5'- cGUCGGG-UCGGCGUCgaccaucgUGCccaacAGGCCGAGa -3' miRNA: 3'- -UAGUCCgAGCCGCAG--------GCG-----UCUGGCUC- -5' |
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| 1467 | 5' | -60.3 | NC_001335.1 | + | 599 | 0.68 | 0.296579 |
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Target: 5'- -aUAGGCcggcacgaagUCGGuCGgCCGCAGAUCGGGg -3' miRNA: 3'- uaGUCCG----------AGCC-GCaGGCGUCUGGCUC- -5' |
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| 1467 | 5' | -60.3 | NC_001335.1 | + | 2086 | 0.71 | 0.192694 |
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Target: 5'- gAUCGGGCUCGGCGgCUGCGuGAacaCGAc -3' miRNA: 3'- -UAGUCCGAGCCGCaGGCGU-CUg--GCUc -5' |
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| 1467 | 5' | -60.3 | NC_001335.1 | + | 4699 | 0.72 | 0.182822 |
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Target: 5'- cUCuGGCcUGGUG-CUGCAGACCGGGg -3' miRNA: 3'- uAGuCCGaGCCGCaGGCGUCUGGCUC- -5' |
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| 1467 | 5' | -60.3 | NC_001335.1 | + | 36897 | 0.75 | 0.100704 |
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Target: 5'- cGUCAGGCUgGGUGUCCccgauGUAG-CCGAGa -3' miRNA: 3'- -UAGUCCGAgCCGCAGG-----CGUCuGGCUC- -5' |
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| 1467 | 5' | -60.3 | NC_001335.1 | + | 14206 | 1.07 | 0.000424 |
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Target: 5'- gAUCAGGCUCGGCGUCCGCAGACCGAGa -3' miRNA: 3'- -UAGUCCGAGCCGCAGGCGUCUGGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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