Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14670 | 3' | -50.1 | NC_003525.1 | + | 2409 | 0.66 | 0.95646 |
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Target: 5'- aCga-CCUGAUgGAGCAGAucgaacguccggAUGGCAUUa -3' miRNA: 3'- gGaagGGACUA-UUCGUCU------------UGCCGUAG- -5' |
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| 14670 | 3' | -50.1 | NC_003525.1 | + | 21862 | 0.66 | 0.95646 |
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Target: 5'- gCUUCCCUGuc-AGUAGAACaGUuguuUCa -3' miRNA: 3'- gGAAGGGACuauUCGUCUUGcCGu---AG- -5' |
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| 14670 | 3' | -50.1 | NC_003525.1 | + | 47143 | 0.66 | 0.937304 |
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Target: 5'- uUCUUCCCUGGU-AGCAaacCGGUAa- -3' miRNA: 3'- -GGAAGGGACUAuUCGUcuuGCCGUag -5' |
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| 14670 | 3' | -50.1 | NC_003525.1 | + | 20679 | 0.68 | 0.899793 |
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Target: 5'- gCUUCCCggcGAUGAGCugcGGAU-GCGUCa -3' miRNA: 3'- gGAAGGGa--CUAUUCGu--CUUGcCGUAG- -5' |
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| 14670 | 3' | -50.1 | NC_003525.1 | + | 23740 | 0.68 | 0.868217 |
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Target: 5'- --aUCCCUGAcGGGCGGAccguucgcgagaaGCuGCGUCa -3' miRNA: 3'- ggaAGGGACUaUUCGUCU-------------UGcCGUAG- -5' |
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| 14670 | 3' | -50.1 | NC_003525.1 | + | 27272 | 0.69 | 0.86068 |
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Target: 5'- aUCUUCUgCUGGUcuGCAu-ACGGCAUCa -3' miRNA: 3'- -GGAAGG-GACUAuuCGUcuUGCCGUAG- -5' |
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| 14670 | 3' | -50.1 | NC_003525.1 | + | 5577 | 0.69 | 0.824775 |
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Target: 5'- aCCUUugaCCCUGAUAGaaaCGGAuCGGCAUa -3' miRNA: 3'- -GGAA---GGGACUAUUc--GUCUuGCCGUAg -5' |
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| 14670 | 3' | -50.1 | NC_003525.1 | + | 38868 | 1.13 | 0.001933 |
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Target: 5'- gCCUUCCCUGAUAAGCAGAACGGCAUCg -3' miRNA: 3'- -GGAAGGGACUAUUCGUCUUGCCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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