Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14671 | 3' | -52.7 | NC_003525.1 | + | 46339 | 0.66 | 0.845831 |
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Target: 5'- cUUUCCCGGcGAGucuggcuaaCCGGGACCagaacgaACUGa -3' miRNA: 3'- aAAAGGGCCuUUC---------GGUCUUGGg------UGAC- -5' |
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| 14671 | 3' | -52.7 | NC_003525.1 | + | 37972 | 0.66 | 0.836854 |
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Target: 5'- gUUUUCCCc--AGGCCAGAGCCUuaGCg- -3' miRNA: 3'- -AAAAGGGccuUUCGGUCUUGGG--UGac -5' |
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| 14671 | 3' | -52.7 | NC_003525.1 | + | 9209 | 0.67 | 0.798778 |
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Target: 5'- --cUCgUGGAGAGCCAGGuCCgGCa- -3' miRNA: 3'- aaaAGgGCCUUUCGGUCUuGGgUGac -5' |
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| 14671 | 3' | -52.7 | NC_003525.1 | + | 22349 | 0.67 | 0.788771 |
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Target: 5'- uUUUUCUCGGAAAGCaGGGAUgaaUUACUGg -3' miRNA: 3'- -AAAAGGGCCUUUCGgUCUUG---GGUGAC- -5' |
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| 14671 | 3' | -52.7 | NC_003525.1 | + | 8826 | 0.67 | 0.778592 |
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Target: 5'- ---gCCCuGcgGGCCcuugAGGGCCCACUGg -3' miRNA: 3'- aaaaGGGcCuuUCGG----UCUUGGGUGAC- -5' |
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| 14671 | 3' | -52.7 | NC_003525.1 | + | 401 | 0.67 | 0.768253 |
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Target: 5'- uUUUUCCCGuGAcAGCUucAGCCCGCUu -3' miRNA: 3'- -AAAAGGGC-CUuUCGGucUUGGGUGAc -5' |
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| 14671 | 3' | -52.7 | NC_003525.1 | + | 8885 | 0.67 | 0.757766 |
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Target: 5'- ----aCCGGAGAGCgGGGGCCUAaaGg -3' miRNA: 3'- aaaagGGCCUUUCGgUCUUGGGUgaC- -5' |
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| 14671 | 3' | -52.7 | NC_003525.1 | + | 46428 | 0.72 | 0.507295 |
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Target: 5'- --gUCCCGGuuAGCCAGAcucgccgggaaaguuGCCaguaACUGg -3' miRNA: 3'- aaaAGGGCCuuUCGGUCU---------------UGGg---UGAC- -5' |
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| 14671 | 3' | -52.7 | NC_003525.1 | + | 46482 | 1.07 | 0.002297 |
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Target: 5'- gUUUUCCCGGAAAGCCAGAACCCACUGg -3' miRNA: 3'- -AAAAGGGCCUUUCGGUCUUGGGUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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