miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
14674 3' -57.6 NC_003525.1 + 15450 0.66 0.618845
Target:  5'- gACUGGCGCacgaguggcguuacuGCUGGCauugGCCugaaauucugaccuGUAUCCGg -3'
miRNA:   3'- -UGGCUGCG---------------UGACCGga--CGG--------------UAUAGGC- -5'
14674 3' -57.6 NC_003525.1 + 630 0.67 0.56202
Target:  5'- aGCgGACGCugaugaauuaucuGCUGGUaugggaaCUGCCGgAUCCGg -3'
miRNA:   3'- -UGgCUGCG-------------UGACCG-------GACGGUaUAGGC- -5'
14674 3' -57.6 NC_003525.1 + 58169 0.67 0.553547
Target:  5'- gACgGugGCAgaGa-CUGCCGUAUCCGc -3'
miRNA:   3'- -UGgCugCGUgaCcgGACGGUAUAGGC- -5'
14674 3' -57.6 NC_003525.1 + 25469 0.68 0.481408
Target:  5'- uGCCcggGAUGCACgccccaUGGCCUGCCugcuuucacGUCCGc -3'
miRNA:   3'- -UGG---CUGCGUG------ACCGGACGGua-------UAGGC- -5'
14674 3' -57.6 NC_003525.1 + 10943 0.7 0.37839
Target:  5'- uCCGcUGUACUGGCggaggaCUGCCAUuUCCGg -3'
miRNA:   3'- uGGCuGCGUGACCG------GACGGUAuAGGC- -5'
14674 3' -57.6 NC_003525.1 + 8126 0.74 0.20407
Target:  5'- uGCCGACGCACUGGUgccgCUGagCGcAUCCGg -3'
miRNA:   3'- -UGGCUGCGUGACCG----GACg-GUaUAGGC- -5'
14674 3' -57.6 NC_003525.1 + 4815 0.75 0.192628
Target:  5'- aACCGGCGCacccgcGCUGGUCUGCUucgugcuuuucuUGUCCGg -3'
miRNA:   3'- -UGGCUGCG------UGACCGGACGGu-----------AUAGGC- -5'
14674 3' -57.6 NC_003525.1 + 55788 0.98 0.004459
Target:  5'- cACCGACGCAC-GGCCUGCCAUAUCCGc -3'
miRNA:   3'- -UGGCUGCGUGaCCGGACGGUAUAGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.