Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14678 | 3' | -57.4 | NC_003525.1 | + | 9440 | 0.68 | 0.530812 |
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Target: 5'- cGGGGCCagCAGGucc-GGCUGGgCCGAa -3' miRNA: 3'- -CCCCGGa-GUCCcucuUUGACCaGGCU- -5' |
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| 14678 | 3' | -57.4 | NC_003525.1 | + | 8895 | 0.68 | 0.520501 |
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Target: 5'- gGGGGCCUaaaggggacCAGGGGGAua-UGGggCCa- -3' miRNA: 3'- -CCCCGGA---------GUCCCUCUuugACCa-GGcu -5' |
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| 14678 | 3' | -57.4 | NC_003525.1 | + | 9203 | 0.7 | 0.431874 |
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Target: 5'- aGGGaCCUCGuGGAGAGcCaGGUCCGGc -3' miRNA: 3'- cCCC-GGAGUcCCUCUUuGaCCAGGCU- -5' |
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| 14678 | 3' | -57.4 | NC_003525.1 | + | 8797 | 0.72 | 0.305913 |
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Target: 5'- gGGGGCUgUAGGGccu-GCUGGUCCGc -3' miRNA: 3'- -CCCCGGaGUCCCucuuUGACCAGGCu -5' |
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| 14678 | 3' | -57.4 | NC_003525.1 | + | 9520 | 0.72 | 0.291411 |
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Target: 5'- cGGGGaCCggCAGGu-GAuGCUGGUCCGGc -3' miRNA: 3'- -CCCC-GGa-GUCCcuCUuUGACCAGGCU- -5' |
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| 14678 | 3' | -57.4 | NC_003525.1 | + | 9574 | 0.78 | 0.133835 |
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Target: 5'- aGGGGagagCGGGGAGAGACcGGUCUGAc -3' miRNA: 3'- -CCCCgga-GUCCCUCUUUGaCCAGGCU- -5' |
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| 14678 | 3' | -57.4 | NC_003525.1 | + | 8679 | 1.11 | 0.000582 |
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Target: 5'- gGGGGCCUCAGGGAGAAACUGGUCCGAa -3' miRNA: 3'- -CCCCGGAGUCCCUCUUUGACCAGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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