Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14678 | 5' | -59.4 | NC_003525.1 | + | 9733 | 0.66 | 0.495212 |
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Target: 5'- ---cGGUCCcuGUGGGCCUgcc-GGGCc -3' miRNA: 3'- uuguCCAGGu-CACCCGGGaguuCCCG- -5' |
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| 14678 | 5' | -59.4 | NC_003525.1 | + | 9130 | 0.66 | 0.465311 |
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Target: 5'- uGCAGGaCCAGguucaCCUCGGGGGCc -3' miRNA: 3'- uUGUCCaGGUCacccgGGAGUUCCCG- -5' |
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| 14678 | 5' | -59.4 | NC_003525.1 | + | 9229 | 0.66 | 0.465311 |
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Target: 5'- uGCAGGaCCAGguucaCCUCGGGGGCc -3' miRNA: 3'- uUGUCCaGGUCacccgGGAGUUCCCG- -5' |
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| 14678 | 5' | -59.4 | NC_003525.1 | + | 9609 | 0.69 | 0.339883 |
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Target: 5'- uGCAGGUCCAcaGGGUCCa-AAGGGa -3' miRNA: 3'- uUGUCCAGGUcaCCCGGGagUUCCCg -5' |
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| 14678 | 5' | -59.4 | NC_003525.1 | + | 8828 | 0.71 | 0.253361 |
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Target: 5'- ---cGGcCCuGcGGGCCCUUGAGGGCc -3' miRNA: 3'- uuguCCaGGuCaCCCGGGAGUUCCCG- -5' |
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| 14678 | 5' | -59.4 | NC_003525.1 | + | 9537 | 0.74 | 0.142139 |
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Target: 5'- uGCuGGUCCGGcaGGCCCgCAGGGGCc -3' miRNA: 3'- uUGuCCAGGUCacCCGGGaGUUCCCG- -5' |
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| 14678 | 5' | -59.4 | NC_003525.1 | + | 9446 | 0.77 | 0.096708 |
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Target: 5'- cAGCAGGUCCGGcUGGGCCg--AAGGGUg -3' miRNA: 3'- -UUGUCCAGGUC-ACCCGGgagUUCCCG- -5' |
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| 14678 | 5' | -59.4 | NC_003525.1 | + | 9689 | 0.8 | 0.051971 |
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Target: 5'- cGGCAGGcCCGGUGGGggcgaccggaCCUCAGGGGCc -3' miRNA: 3'- -UUGUCCaGGUCACCCg---------GGAGUUCCCG- -5' |
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| 14678 | 5' | -59.4 | NC_003525.1 | + | 8730 | 1.08 | 0.000462 |
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Target: 5'- aAACAGGUCCAGUGGGCCCUCAAGGGCc -3' miRNA: 3'- -UUGUCCAGGUCACCCGGGAGUUCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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