Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1468 | 3' | -52.3 | NC_001335.1 | + | 34513 | 0.72 | 0.601165 |
Target: 5'- cGAUCUgAUCGAC-ACGcacuaccgacccggCACGGUCCa -3' miRNA: 3'- cCUAGGgUAGCUGaUGCa-------------GUGCCAGG- -5' |
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1468 | 3' | -52.3 | NC_001335.1 | + | 29139 | 0.72 | 0.579237 |
Target: 5'- cGGucuUCCCAaccuuucgcggcaccUgGGCUGCGUCAgCGGUCa -3' miRNA: 3'- -CCu--AGGGU---------------AgCUGAUGCAGU-GCCAGg -5' |
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1468 | 3' | -52.3 | NC_001335.1 | + | 14519 | 1.15 | 0.001033 |
Target: 5'- aGGAUCCCAUCGACUACGUCACGGUCCu -3' miRNA: 3'- -CCUAGGGUAGCUGAUGCAGUGCCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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