miRNA: 3'- gUCaAGCUACUCGUC--AGCGac--CCAGa -5'
miRNA: 3'- gUCA---AGCUACUCGUcAGCGAc--CCAGA- -5'
miRNA: 3'- gUCA--AGCUACUCGU-C-AGCGa------------CCCAGa -5'
miRNA: 3'- gucaAGCUACUCGUC-AGCGACC-CAGa -5'
miRNA: 3'- -GUCAAGCUACUCGUCAGCGACCCAGA- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1468 | 5' | -54 | NC_001335.1 | + | 49464 | 0.67 | 0.674356 |
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Target: 5'- uGGcUCGGUGGGCAGccUCGCggaaGGUCa -3' miRNA: 3'- gUCaAGCUACUCGUC--AGCGac--CCAGa -5' |
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| 1468 | 5' | -54 | NC_001335.1 | + | 15170 | 0.68 | 0.640798 |
|
Target: 5'- gAGUuggUCGAUGAGUAcUCGCUccaGGUCUg -3' miRNA: 3'- gUCA---AGCUACUCGUcAGCGAc--CCAGA- -5' |
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| 1468 | 5' | -54 | NC_001335.1 | + | 35455 | 0.69 | 0.591521 |
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Target: 5'- gGGUucUCGAUGAGCAuGaUCGCgauguucgcugugGGGUCa -3' miRNA: 3'- gUCA--AGCUACUCGU-C-AGCGa------------CCCAGa -5' |
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| 1468 | 5' | -54 | NC_001335.1 | + | 3634 | 0.7 | 0.508412 |
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Target: 5'- ---cUCGGUGAGCGGcUUGCUGGcGUUg -3' miRNA: 3'- gucaAGCUACUCGUC-AGCGACC-CAGa -5' |
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| 1468 | 5' | -54 | NC_001335.1 | + | 14553 | 1.08 | 0.001327 |
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Target: 5'- cCAGUUCGAUGAGCAGUCGCUGGGUCUg -3' miRNA: 3'- -GUCAAGCUACUCGUCAGCGACCCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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