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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14683 | 3' | -57.4 | NC_003525.1 | + | 56310 | 0.66 | 0.627323 |
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Target: 5'- -uGGAGUACGCCG-CGGGCaaCGGugcGGc -3' miRNA: 3'- guCUUCAUGCGGCuGUCCG--GCCu--CCu -5' |
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| 14683 | 3' | -57.4 | NC_003525.1 | + | 38069 | 0.68 | 0.531673 |
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Target: 5'- cCGGAAGUuaACGCUaaGGCucuGGCCuGGGGAa -3' miRNA: 3'- -GUCUUCA--UGCGG--CUGu--CCGGcCUCCU- -5' |
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| 14683 | 3' | -57.4 | NC_003525.1 | + | 21394 | 0.69 | 0.460077 |
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Target: 5'- gAGAAGUggcgcguACGCCGGaugaaaauCAGGCCGGAcguGGu -3' miRNA: 3'- gUCUUCA-------UGCGGCU--------GUCCGGCCU---CCu -5' |
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| 14683 | 3' | -57.4 | NC_003525.1 | + | 22047 | 0.7 | 0.413763 |
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Target: 5'- -cGAAG-ACGaaacacaauaCGGCAGGCUGGGGGGa -3' miRNA: 3'- guCUUCaUGCg---------GCUGUCCGGCCUCCU- -5' |
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| 14683 | 3' | -57.4 | NC_003525.1 | + | 21705 | 1.08 | 0.000808 |
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Target: 5'- gCAGAAGUACGCCGACAGGCCGGAGGAa -3' miRNA: 3'- -GUCUUCAUGCGGCUGUCCGGCCUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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