Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14686 | 5' | -57.7 | NC_003525.1 | + | 61561 | 0.66 | 0.622817 |
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Target: 5'- aUGGcUGUGGAaaaucCCAGCuGGCGCGCUUu -3' miRNA: 3'- -ACU-ACGCUUac---GGUCG-CCGCGCGAGc -5' |
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| 14686 | 5' | -57.7 | NC_003525.1 | + | 25072 | 0.66 | 0.619572 |
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Target: 5'- ---cGCGAaaaucaacccgaaaAUGCCGGaCGGCG-GUUCGg -3' miRNA: 3'- acuaCGCU--------------UACGGUC-GCCGCgCGAGC- -5' |
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| 14686 | 5' | -57.7 | NC_003525.1 | + | 31933 | 0.66 | 0.579703 |
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Target: 5'- aUGGUGCauGGAUGCCugaguGCaGCGUGaCUCa -3' miRNA: 3'- -ACUACG--CUUACGGu----CGcCGCGC-GAGc -5' |
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| 14686 | 5' | -57.7 | NC_003525.1 | + | 54674 | 0.67 | 0.558365 |
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Target: 5'- aUGGUGcCGggUGCCuccCGGUGaGUUCGg -3' miRNA: 3'- -ACUAC-GCuuACGGuc-GCCGCgCGAGC- -5' |
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| 14686 | 5' | -57.7 | NC_003525.1 | + | 61667 | 0.67 | 0.537254 |
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Target: 5'- ---aGCG-GUGCCAGCGGUG-GCUa- -3' miRNA: 3'- acuaCGCuUACGGUCGCCGCgCGAgc -5' |
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| 14686 | 5' | -57.7 | NC_003525.1 | + | 20175 | 0.68 | 0.49593 |
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Target: 5'- gGAU-CGGuUGCCAGCuGCGCGCg-- -3' miRNA: 3'- aCUAcGCUuACGGUCGcCGCGCGagc -5' |
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| 14686 | 5' | -57.7 | NC_003525.1 | + | 7812 | 0.69 | 0.418066 |
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Target: 5'- gGAUGC---UGCCGGUGGCGCagaaUCGg -3' miRNA: 3'- aCUACGcuuACGGUCGCCGCGcg--AGC- -5' |
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| 14686 | 5' | -57.7 | NC_003525.1 | + | 35829 | 1.09 | 0.000571 |
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Target: 5'- uUGAUGCGAAUGCCAGCGGCGCGCUCGg -3' miRNA: 3'- -ACUACGCUUACGGUCGCCGCGCGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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