Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14688 | 3' | -50.7 | NC_003525.1 | + | 28012 | 0.68 | 0.897008 |
Target: 5'- cCGUCCUUCAGGGCGu-GGAGGaugUCa -3' miRNA: 3'- cGUAGGGAGUUUUGCucCCUCCa--AGg -5' |
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14688 | 3' | -50.7 | NC_003525.1 | + | 45707 | 0.69 | 0.831757 |
Target: 5'- uGCuAUCCCUCAAAAC-AGGGGGa---- -3' miRNA: 3'- -CG-UAGGGAGUUUUGcUCCCUCcaagg -5' |
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14688 | 3' | -50.7 | NC_003525.1 | + | 27148 | 0.71 | 0.720771 |
Target: 5'- aGCAggugCCCUUccuggggaAAAuuuCGGGGGAGGUgaugCCg -3' miRNA: 3'- -CGUa---GGGAG--------UUUu--GCUCCCUCCAa---GG- -5' |
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14688 | 3' | -50.7 | NC_003525.1 | + | 45642 | 0.85 | 0.133184 |
Target: 5'- aCAUCCCUCAAAACGAGGGAaaaUCCc -3' miRNA: 3'- cGUAGGGAGUUUUGCUCCCUccaAGG- -5' |
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14688 | 3' | -50.7 | NC_003525.1 | + | 45602 | 1.14 | 0.001792 |
Target: 5'- uGCAUCCCUCAAAACGAGGGAGGUUCCc -3' miRNA: 3'- -CGUAGGGAGUUUUGCUCCCUCCAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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