Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14689 | 5' | -55.4 | NC_003525.1 | + | 8120 | 0.7 | 0.46268 |
Target: 5'- cGCAcUGGUGCCGcuGAGc-GCAUCCGg -3' miRNA: 3'- aCGU-ACCACGGCccUUCuuCGUAGGC- -5' |
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14689 | 5' | -55.4 | NC_003525.1 | + | 46510 | 1.09 | 0.001097 |
Target: 5'- uUGCAUGGUGCCGGGAAGAAGCAUCCGu -3' miRNA: 3'- -ACGUACCACGGCCCUUCUUCGUAGGC- -5' |
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14689 | 5' | -55.4 | NC_003525.1 | + | 52307 | 0.7 | 0.452758 |
Target: 5'- aGCGUGaauUGCCGGGAGGGAGaaccUCUGu -3' miRNA: 3'- aCGUACc--ACGGCCCUUCUUCgu--AGGC- -5' |
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14689 | 5' | -55.4 | NC_003525.1 | + | 54678 | 0.74 | 0.268219 |
Target: 5'- cUGCAUGGUGCCGGGu----GCcUCCc -3' miRNA: 3'- -ACGUACCACGGCCCuucuuCGuAGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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