Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14690 | 3' | -51.7 | NC_003525.1 | + | 29728 | 0.66 | 0.900482 |
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Target: 5'- aGCA--AUGGCUUCaggcGUGCGaCGGAa -3' miRNA: 3'- gCGUauUAUCGAAGa---CACGCgGCCUg -5' |
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| 14690 | 3' | -51.7 | NC_003525.1 | + | 56209 | 0.66 | 0.870008 |
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Target: 5'- aCGCcu-GUGGCUUCUGcaccggaagGCGaugaCGGGCg -3' miRNA: 3'- -GCGuauUAUCGAAGACa--------CGCg---GCCUG- -5' |
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| 14690 | 3' | -51.7 | NC_003525.1 | + | 51044 | 0.66 | 0.870008 |
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Target: 5'- uGCAU--UGGCacau-UGCGCCGGGCa -3' miRNA: 3'- gCGUAuuAUCGaagacACGCGGCCUG- -5' |
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| 14690 | 3' | -51.7 | NC_003525.1 | + | 48258 | 0.67 | 0.826125 |
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Target: 5'- gGCAUGGUcaacGGCUg----GCGCCGGAa -3' miRNA: 3'- gCGUAUUA----UCGAagacaCGCGGCCUg -5' |
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| 14690 | 3' | -51.7 | NC_003525.1 | + | 54153 | 0.7 | 0.701637 |
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Target: 5'- uGCAU--UAGCUUCUGUuaaugcaaugGCGgCGGAUu -3' miRNA: 3'- gCGUAuuAUCGAAGACA----------CGCgGCCUG- -5' |
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| 14690 | 3' | -51.7 | NC_003525.1 | + | 20940 | 0.73 | 0.511755 |
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Target: 5'- aCGCAUug-AGaag--GUGCGCCGGACa -3' miRNA: 3'- -GCGUAuuaUCgaagaCACGCGGCCUG- -5' |
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| 14690 | 3' | -51.7 | NC_003525.1 | + | 46924 | 1.12 | 0.001525 |
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Target: 5'- aCGCAUAAUAGCUUCUGUGCGCCGGACg -3' miRNA: 3'- -GCGUAUUAUCGAAGACACGCGGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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