Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14695 | 3' | -49.3 | NC_003529.1 | + | 53366 | 0.66 | 0.997575 |
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Target: 5'- cGUGCAucucugGAAUGCCgccgGUGCUAGUc- -3' miRNA: 3'- cCACGU------UUUACGGaug-CACGGUCAuu -5' |
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| 14695 | 3' | -49.3 | NC_003529.1 | + | 85568 | 0.66 | 0.997575 |
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Target: 5'- cGGU-CGGAAUGCCg--GUGCCGGc-- -3' miRNA: 3'- -CCAcGUUUUACGGaugCACGGUCauu -5' |
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| 14695 | 3' | -49.3 | NC_003529.1 | + | 21844 | 0.66 | 0.995951 |
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Target: 5'- gGGguggGCgAAAGUGCCcgGCGUGCCcAGc-- -3' miRNA: 3'- -CCa---CG-UUUUACGGa-UGCACGG-UCauu -5' |
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| 14695 | 3' | -49.3 | NC_003529.1 | + | 114568 | 0.67 | 0.99353 |
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Target: 5'- --cGCGAuuGUGCCUugGUGCUgAGUu- -3' miRNA: 3'- ccaCGUUu-UACGGAugCACGG-UCAuu -5' |
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| 14695 | 3' | -49.3 | NC_003529.1 | + | 117551 | 0.67 | 0.992504 |
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Target: 5'- gGGUGCGuuGGUGCCca-GUGCCgAGUu- -3' miRNA: 3'- -CCACGUu-UUACGGaugCACGG-UCAuu -5' |
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| 14695 | 3' | -49.3 | NC_003529.1 | + | 21735 | 0.67 | 0.992504 |
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Target: 5'- --aGCAGGAcgGUggACGUGCCGGUAu -3' miRNA: 3'- ccaCGUUUUa-CGgaUGCACGGUCAUu -5' |
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| 14695 | 3' | -49.3 | NC_003529.1 | + | 27606 | 0.7 | 0.960495 |
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Target: 5'- -uUGUaAAAAUGCgUGCgGUGCCGGUGAg -3' miRNA: 3'- ccACG-UUUUACGgAUG-CACGGUCAUU- -5' |
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| 14695 | 3' | -49.3 | NC_003529.1 | + | 68920 | 0.71 | 0.933119 |
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Target: 5'- cGGUGCcGAAUGCgUugGcGCCGGa-- -3' miRNA: 3'- -CCACGuUUUACGgAugCaCGGUCauu -5' |
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| 14695 | 3' | -49.3 | NC_003529.1 | + | 17092 | 1.1 | 0.009641 |
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Target: 5'- aGGUGCAAAAUGCCUACGUGCCAGUAAa -3' miRNA: 3'- -CCACGUUUUACGGAUGCACGGUCAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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