Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14695 | 5' | -48.7 | NC_003529.1 | + | 68921 | 0.66 | 0.998683 |
Target: 5'- -----gUGGCGUACGgccgGcaACGCgAUGCg -3' miRNA: 3'- auguuaACCGCAUGCa---C--UGUGgUACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 21693 | 0.66 | 0.998076 |
Target: 5'- gGCGAUcuUGGCG-AUGUGGCAUacgucuUGCu -3' miRNA: 3'- aUGUUA--ACCGCaUGCACUGUGgu----ACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 42797 | 0.67 | 0.996735 |
Target: 5'- cACGGUcUGGUGgacACGcUGGCugUAUGCg -3' miRNA: 3'- aUGUUA-ACCGCa--UGC-ACUGugGUACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 46384 | 0.67 | 0.996391 |
Target: 5'- aAUAGUUGGCGUACGUcuugagaaugucgcGCACUuggucccgAUGCg -3' miRNA: 3'- aUGUUAACCGCAUGCAc-------------UGUGG--------UACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 41224 | 0.67 | 0.996145 |
Target: 5'- -----aUGGUGUGUGUGcaGCGCCGUGUa -3' miRNA: 3'- auguuaACCGCAUGCAC--UGUGGUACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 152544 | 0.68 | 0.994703 |
Target: 5'- aGCAAUugUGGC-UACcaGAgCACCAUGCa -3' miRNA: 3'- aUGUUA--ACCGcAUGcaCU-GUGGUACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 102850 | 0.68 | 0.992852 |
Target: 5'- -uCGAUa-GUGUACGUGGCAUCAaUGCg -3' miRNA: 3'- auGUUAacCGCAUGCACUGUGGU-ACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 37875 | 0.68 | 0.99175 |
Target: 5'- cACGGuUUGcGCGUGCGcgaaaUGGC-CCGUGCu -3' miRNA: 3'- aUGUU-AAC-CGCAUGC-----ACUGuGGUACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 13946 | 0.7 | 0.98206 |
Target: 5'- -----aUGGCGUcguAgGUGACGCCuGUGCu -3' miRNA: 3'- auguuaACCGCA---UgCACUGUGG-UACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 114885 | 0.7 | 0.97984 |
Target: 5'- cUGCAcGUUGGCGUAaaacGAUGCCGUGUu -3' miRNA: 3'- -AUGU-UAACCGCAUgca-CUGUGGUACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 45297 | 0.71 | 0.968894 |
Target: 5'- cACAGac-GCGUuuccCGUGGCACCAUGUc -3' miRNA: 3'- aUGUUaacCGCAu---GCACUGUGGUACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 141612 | 0.71 | 0.968894 |
Target: 5'- aACG--UGGUGUACGUGAa--CAUGCc -3' miRNA: 3'- aUGUuaACCGCAUGCACUgugGUACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 98501 | 0.71 | 0.965599 |
Target: 5'- cGCGA-UGGCGUGCaccGACACUggGCg -3' miRNA: 3'- aUGUUaACCGCAUGca-CUGUGGuaCG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 137782 | 0.71 | 0.965599 |
Target: 5'- cACGuaaaUGGCGUauuuguAUGUGACGCCA-GCg -3' miRNA: 3'- aUGUua--ACCGCA------UGCACUGUGGUaCG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 133282 | 0.71 | 0.957897 |
Target: 5'- gGCAAUgguugcgUGcGCGuUACGUGAUAgCGUGCa -3' miRNA: 3'- aUGUUA-------AC-CGC-AUGCACUGUgGUACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 66881 | 0.71 | 0.954261 |
Target: 5'- cACGAUuUGGUGUGCaugGUGACGCCugAUGUc -3' miRNA: 3'- aUGUUA-ACCGCAUG---CACUGUGG--UACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 111553 | 0.78 | 0.699542 |
Target: 5'- aUACGGUUGGUGUACcguggGUGACAUgAUGUg -3' miRNA: 3'- -AUGUUAACCGCAUG-----CACUGUGgUACG- -5' |
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14695 | 5' | -48.7 | NC_003529.1 | + | 17058 | 1.12 | 0.009028 |
Target: 5'- cUACAAUUGGCGUACGUGACACCAUGCa -3' miRNA: 3'- -AUGUUAACCGCAUGCACUGUGGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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