Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14696 | 5' | -55.1 | NC_003529.1 | + | 105731 | 0.66 | 0.941205 |
Target: 5'- -----aUCGCGGaGUGCgauagCGGuCGCCCa -3' miRNA: 3'- acuugaAGCGCCgUACGa----GCU-GCGGG- -5' |
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14696 | 5' | -55.1 | NC_003529.1 | + | 145445 | 0.66 | 0.926736 |
Target: 5'- aGGAUUguugcUCGCGGCuacauUGaaagcgggaacaucaUCGAUGCCCa -3' miRNA: 3'- aCUUGA-----AGCGCCGu----ACg--------------AGCUGCGGG- -5' |
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14696 | 5' | -55.1 | NC_003529.1 | + | 36601 | 0.67 | 0.914414 |
Target: 5'- cGAccGCUUCGaCGGCuucgucgGUGCUuugcaCGcCGCCCu -3' miRNA: 3'- aCU--UGAAGC-GCCG-------UACGA-----GCuGCGGG- -5' |
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14696 | 5' | -55.1 | NC_003529.1 | + | 107354 | 0.67 | 0.90904 |
Target: 5'- -------gGCGGCAaagUGCUUG-CGCCCa -3' miRNA: 3'- acuugaagCGCCGU---ACGAGCuGCGGG- -5' |
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14696 | 5' | -55.1 | NC_003529.1 | + | 5962 | 0.67 | 0.902845 |
Target: 5'- gGAGCUUCGauGCGggaccacgaaCUCGACGCUa -3' miRNA: 3'- aCUUGAAGCgcCGUac--------GAGCUGCGGg -5' |
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14696 | 5' | -55.1 | NC_003529.1 | + | 66217 | 0.67 | 0.896415 |
Target: 5'- uUGAGCcUUG-GGCAUuuUUGACGCCUg -3' miRNA: 3'- -ACUUGaAGCgCCGUAcgAGCUGCGGG- -5' |
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14696 | 5' | -55.1 | NC_003529.1 | + | 94414 | 0.68 | 0.875754 |
Target: 5'- cGAACcugUCGCGcCAUGuUUCGgucGCGCCCc -3' miRNA: 3'- aCUUGa--AGCGCcGUAC-GAGC---UGCGGG- -5' |
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14696 | 5' | -55.1 | NC_003529.1 | + | 143405 | 0.68 | 0.85313 |
Target: 5'- aGAGCUgcgcCGCGGCAUGUcgguguacggUCGcgagcaguUGCCCu -3' miRNA: 3'- aCUUGAa---GCGCCGUACG----------AGCu-------GCGGG- -5' |
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14696 | 5' | -55.1 | NC_003529.1 | + | 137809 | 0.68 | 0.85313 |
Target: 5'- ---gUUUCGCaGCAguccuUGCaCGACGCCCa -3' miRNA: 3'- acuuGAAGCGcCGU-----ACGaGCUGCGGG- -5' |
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14696 | 5' | -55.1 | NC_003529.1 | + | 93470 | 0.69 | 0.837033 |
Target: 5'- -cAAUUUCGUGGacgGCUCGuCGCUCg -3' miRNA: 3'- acUUGAAGCGCCguaCGAGCuGCGGG- -5' |
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14696 | 5' | -55.1 | NC_003529.1 | + | 143223 | 0.69 | 0.820189 |
Target: 5'- uUGAGCaUCGcCGGCAUGaucuacgUUGGCGCaCCu -3' miRNA: 3'- -ACUUGaAGC-GCCGUACg------AGCUGCG-GG- -5' |
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14696 | 5' | -55.1 | NC_003529.1 | + | 22483 | 0.7 | 0.793657 |
Target: 5'- uUGAGCUUUG-GGCAUuuUUGACGCUUg -3' miRNA: 3'- -ACUUGAAGCgCCGUAcgAGCUGCGGG- -5' |
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14696 | 5' | -55.1 | NC_003529.1 | + | 44061 | 0.7 | 0.756277 |
Target: 5'- uUGcAAUUgCGCGGCAcgcUGCUCGugGUgCa -3' miRNA: 3'- -AC-UUGAaGCGCCGU---ACGAGCugCGgG- -5' |
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14696 | 5' | -55.1 | NC_003529.1 | + | 148079 | 0.76 | 0.466934 |
Target: 5'- cGAuaACUUCG-GGUGUGCUCGGCGCa- -3' miRNA: 3'- aCU--UGAAGCgCCGUACGAGCUGCGgg -5' |
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14696 | 5' | -55.1 | NC_003529.1 | + | 20889 | 1.12 | 0.002384 |
Target: 5'- gUGAACUUCGCGGCAUGCUCGACGCCCu -3' miRNA: 3'- -ACUUGAAGCGCCGUACGAGCUGCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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