Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14698 | 3' | -46.3 | NC_003529.1 | + | 141166 | 0.66 | 0.999953 |
Target: 5'- uUGaCAUAGUUgAGGUg-AUGUGCGuCGCu -3' miRNA: 3'- -AC-GUAUUAA-UCCGgaUACACGUuGCG- -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 134679 | 0.66 | 0.999937 |
Target: 5'- uUGUAUAGUUGGGCCaacUGUGaauaguuuacaUGUaucuGAUGCg -3' miRNA: 3'- -ACGUAUUAAUCCGG---AUAC-----------ACG----UUGCG- -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 44134 | 0.66 | 0.999937 |
Target: 5'- gGCGUAAUagcgaGGGUCUucg-GCGAUGCa -3' miRNA: 3'- aCGUAUUAa----UCCGGAuacaCGUUGCG- -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 34210 | 0.66 | 0.99989 |
Target: 5'- aGCAUG--UGGuGCCUcauaauuUGUGCGcCGCg -3' miRNA: 3'- aCGUAUuaAUC-CGGAu------ACACGUuGCG- -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 68923 | 0.66 | 0.99989 |
Target: 5'- gGCGUAc---GGCCg----GCAACGCg -3' miRNA: 3'- aCGUAUuaauCCGGauacaCGUUGCG- -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 133525 | 0.66 | 0.99988 |
Target: 5'- cGCAUGucgugauccgucauAUUGGGCCUG-GUGacuugGGCGUa -3' miRNA: 3'- aCGUAU--------------UAAUCCGGAUaCACg----UUGCG- -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 45514 | 0.66 | 0.999855 |
Target: 5'- --------cAGGCCUGUGUGaucuaAGCGUa -3' miRNA: 3'- acguauuaaUCCGGAUACACg----UUGCG- -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 61521 | 0.67 | 0.999812 |
Target: 5'- gGCAguUAAUU--GCCgacaaGUGCAGCGCa -3' miRNA: 3'- aCGU--AUUAAucCGGaua--CACGUUGCG- -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 104908 | 0.67 | 0.999812 |
Target: 5'- gGCAaAAUUuaucAGGCCUAUGaugaaauaacauUGCAGCa- -3' miRNA: 3'- aCGUaUUAA----UCCGGAUAC------------ACGUUGcg -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 25268 | 0.67 | 0.999689 |
Target: 5'- cGcCAUAAUUGGGguggaaaCUAUcGUGCAACcGCu -3' miRNA: 3'- aC-GUAUUAAUCCg------GAUA-CACGUUG-CG- -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 96108 | 0.69 | 0.998242 |
Target: 5'- cUGCGaaugguguUGAUgAGGCCUAgcaUGUGCggUGUc -3' miRNA: 3'- -ACGU--------AUUAaUCCGGAU---ACACGuuGCG- -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 558 | 0.69 | 0.996957 |
Target: 5'- uUGUAacaacGGUUGGGUCUA-GUGgGACGCa -3' miRNA: 3'- -ACGUa----UUAAUCCGGAUaCACgUUGCG- -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 10538 | 0.7 | 0.996385 |
Target: 5'- cGCAaacUAGUUuGGCUcgUAUGUGCGACa- -3' miRNA: 3'- aCGU---AUUAAuCCGG--AUACACGUUGcg -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 75402 | 0.71 | 0.990829 |
Target: 5'- gGCGUGA-UGGGCaugccgggugcgCUGUGUGCAcuCGCu -3' miRNA: 3'- aCGUAUUaAUCCG------------GAUACACGUu-GCG- -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 41214 | 0.73 | 0.974921 |
Target: 5'- aGgAUGGgggauGGUgUGUGUGCAGCGCc -3' miRNA: 3'- aCgUAUUaau--CCGgAUACACGUUGCG- -5' |
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14698 | 3' | -46.3 | NC_003529.1 | + | 30300 | 1.01 | 0.07248 |
Target: 5'- aUGCAUAAUauGGCCUAUGUGCAACGCu -3' miRNA: 3'- -ACGUAUUAauCCGGAUACACGUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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