Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14700 | 3' | -52.9 | NC_003529.1 | + | 53273 | 0.66 | 0.977265 |
Target: 5'- cCGAU-UUG-CAggGGUGuCGUGGCCAu -3' miRNA: 3'- -GCUGuAACuGUuaCCACcGCACCGGU- -5' |
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14700 | 3' | -52.9 | NC_003529.1 | + | 3251 | 0.66 | 0.969007 |
Target: 5'- gCGaACGUUGAC---GGUGGCGUuauuuuGGUCAa -3' miRNA: 3'- -GC-UGUAACUGuuaCCACCGCA------CCGGU- -5' |
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14700 | 3' | -52.9 | NC_003529.1 | + | 31693 | 0.66 | 0.965832 |
Target: 5'- aCGGCAaUGGCGAUGG-GGCcaacaUGGCg- -3' miRNA: 3'- -GCUGUaACUGUUACCaCCGc----ACCGgu -5' |
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14700 | 3' | -52.9 | NC_003529.1 | + | 143710 | 0.66 | 0.965832 |
Target: 5'- uGGCAUUGugaaacacgGCAAUGGc-GCGUGGCa- -3' miRNA: 3'- gCUGUAAC---------UGUUACCacCGCACCGgu -5' |
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14700 | 3' | -52.9 | NC_003529.1 | + | 1388 | 0.67 | 0.962434 |
Target: 5'- cCGGCGgaggUGGCGGcGGUGGCGgcggUGGUUg -3' miRNA: 3'- -GCUGUa---ACUGUUaCCACCGC----ACCGGu -5' |
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14700 | 3' | -52.9 | NC_003529.1 | + | 32776 | 0.67 | 0.961371 |
Target: 5'- cCGGCAggu-CGGUGGUGGCGUcgaguuccuugggcGGCaCAa -3' miRNA: 3'- -GCUGUaacuGUUACCACCGCA--------------CCG-GU- -5' |
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14700 | 3' | -52.9 | NC_003529.1 | + | 24872 | 0.67 | 0.950853 |
Target: 5'- ------aGACGG-GGUGGCG-GGCCAa -3' miRNA: 3'- gcuguaaCUGUUaCCACCGCaCCGGU- -5' |
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14700 | 3' | -52.9 | NC_003529.1 | + | 92706 | 0.67 | 0.946514 |
Target: 5'- gGugGUggcGGCGGUGGUGGCgGUGGa-- -3' miRNA: 3'- gCugUAa--CUGUUACCACCG-CACCggu -5' |
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14700 | 3' | -52.9 | NC_003529.1 | + | 82891 | 0.67 | 0.946514 |
Target: 5'- cCGACAccaACGAaccGGUGGCGUGuGCCc -3' miRNA: 3'- -GCUGUaacUGUUa--CCACCGCAC-CGGu -5' |
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14700 | 3' | -52.9 | NC_003529.1 | + | 105272 | 0.68 | 0.921109 |
Target: 5'- aCGGCGgcuGCGGUGGUGGUgGUGGUUg -3' miRNA: 3'- -GCUGUaacUGUUACCACCG-CACCGGu -5' |
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14700 | 3' | -52.9 | NC_003529.1 | + | 68901 | 0.7 | 0.851963 |
Target: 5'- cCGACAcuUUcGAUGGguUGGUGGCGUacGGCCGg -3' miRNA: 3'- -GCUGU--AA-CUGUU--ACCACCGCA--CCGGU- -5' |
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14700 | 3' | -52.9 | NC_003529.1 | + | 21857 | 0.72 | 0.790918 |
Target: 5'- uGAUGUUGACGAUGG-GGUG-GGCgAa -3' miRNA: 3'- gCUGUAACUGUUACCaCCGCaCCGgU- -5' |
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14700 | 3' | -52.9 | NC_003529.1 | + | 3635 | 0.73 | 0.732653 |
Target: 5'- uCGGCGguggUGAUuguagguGUGGUGGuCGUGGUCAg -3' miRNA: 3'- -GCUGUa---ACUGu------UACCACC-GCACCGGU- -5' |
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14700 | 3' | -52.9 | NC_003529.1 | + | 40030 | 1.09 | 0.005396 |
Target: 5'- cCGACAUUGACAAUGGUGGCGUGGCCAu -3' miRNA: 3'- -GCUGUAACUGUUACCACCGCACCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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